肿瘤药敏试验的最新研究进展  

Recent progress in chemosensitivity test of tumor

作　　者：王杰钦 游辅宇 彭青[1] 高毅[1] WANG Jieqin;YOU Fuyu;PENG Qing;GAO Yi(State Key Laboratory of Organ Failure Research,Second Department of Hepatobiliary Surgery,Zhujiang Hospital,Southern Medical University,Guangzhou 510282,Guangdong Province,China)

机构地区：[1]南方医科大学珠江医院肝胆二科器官衰竭防治国家重点实验室,广东广州510282

出　　处：《肿瘤》2018年第9期894-900,共7页Tumor

基　　金：国家自然科学基金资助项目(编号:81470875);广东省自然科学基金资助项目(编号:2014A030312013);广东省科技计划项目(编号:2015B020229002)~~

摘　　要：肿瘤是威胁人类健康的一大"杀手",迄今为止尚未有十分有效的治疗方法。化疗是肿瘤非手术治疗中最常用的手段,然而由于肿瘤异质性的广泛存在,肿瘤多药耐药和治疗失败的案例比比皆是。为了避免化疗的盲目性,并提高化疗的有效率,基于肿瘤药敏试验的肿瘤个体化治疗方法应运而生。经过半个多世纪的演变,肿瘤药敏试验新方法层出不穷,总体呈现简单、快速、准确和可靠的发展趋势。国内外已先后开展了一系列临床试验,证实肿瘤药敏试验对于肿瘤个体化治疗具有重要的指导意义。然而既往的文献并未将肿瘤药敏试验所涉及的培养方法和检测方法区分开来,容易造成概念混淆。因此,本文重新梳理了当下最为常用的几种肿瘤药敏试验方法,并着重介绍了三维培养法以及新兴的生物发光法、荧光分析法和细胞电阻抗传感器技术等检测方法,以期更全面、更深入地了解肿瘤药敏试验的最新研究进展。Cancer is one of the major ＂killers＂ threatening human health, but so far there is not very effective treatment. Although chemotherapy is the most commonly used for the non-surgical treatment of cancer, due to the extensive presence of tumor heterogeneity, many cases of multidrug resistance and the failure in treatment of tumors can be found everywhere. In order to avoid the blindness of chemotherapy and improve the efficiency of chemotherapy, the personalized therapy of cancer based on chemosensitivity test has come into being. After more than half a century of evolution, the new methods of chemosensitivity test have been developed continuously, with a simple, rapid, accurate and reliable trend. A series of clinical studies have confirmed the importance of chemosensitivity test in guiding personalized therapy of cancer. However, the previous literatures have not distinguished the methods of culture and detection involved in chemosensitivity test, which is easy to cause conceptual confusion. Hence, the most commonly used methods of chemosensitivity test are reorganized in this article, with emphases on the introduction of three-dimensional cell culture, bioluminescence assay, fluorescence analysis and electric cell-substrate impedance sensing, to have a more comprehensive and in-depth understanding of chemosensitivity test.

关 键 词：药物筛选试验 抗肿瘤 精准医学 细胞培养技术 

分 类 号：R965.1[医药卫生—药理学]