钛颗粒刺激小鼠颅骨诱导骨溶解的动物模型建立  

The establishment of an animal model of osteolysis induced by titanium particles in murine calvaria

作　　者：陈德胜[1] 李燕[2] 郭凤英[2] 赵江博 田佳宁 夏根[1] 朱禧[1] CHEN Desheng;LI Yah;GUO Fengying;ZHAO Jiangbo;TIAN Jianing;XIA Gen;ZHU Xi(The General Hospital of Ningxia Medical University,Yinehuan 750004,China;Ningxia Medical University,Yinehuan 750004,China)

机构地区：[1]宁夏医科大学总医院骨科,宁夏银川750004 [2]宁夏医科大学,宁夏银川750004

出　　处：《宁夏医学杂志》2018年第8期677-680,I0001,共5页Ningxia Medical Journal

基　　金：国家自然科学基金项目(81560364);国家自然科学基金项目(81760405);国家自然科学基金项目(81760395);宁夏人事厅留学资助基金项目(2017659)

摘　　要：目的采用钛颗粒刺激小鼠颅骨诱导并建立骨溶解动物模型。方法将30只雌性BALB/c小鼠分为对照组和模型组,每组15只。模型组在小鼠颅顶部切开显露骨膜,将钛颗粒混合液0.3 mL注入骨膜缺损区;对照组切开显露颅骨骨膜,将0.3 mL无菌PBS液注入骨膜缺损区。14 d后分别进行大体下、组织病理学及抗酒石酸酸性染色观察。结果大体下观察小鼠颅骨骨面,对照组完整,色泽光润;模型组可见钛颗粒侵袭骨面,色泽灰暗,并有骨吸收表现。H-E染色观察,对照组小鼠颅骨骨吸收不显著,骨小梁分布均匀,排列规律,骨小梁宽厚稠密,未见有钛颗粒;模型组颅骨骨吸收明显,细胞形态不规则,骨小梁稀疏紊乱,间距增宽,有中断现象。骨吸收面积,对照组为(0.098±0.012)mm2,模型组为(0.365±0.113)mm2,2组比较差异有统计学意义(P<0.05)。TRAP染色,观察对照组小鼠染色较浅,阳性染色区域面积较小,细胞数目不多;模型组染色较深,阳性染色区域面积较大,细胞数目明显增多,有骨吸收的表现。TRAP染色阳性细胞计数,对照组小鼠为(11.42±1.21)个/视野,模型组为(27.82±0.24)个/视野,2组比较差异有统计学意义(P<0.05)。结论钛颗粒刺激小鼠颅骨诱导并建立骨溶解模型,操作简单、经济实用、可重复性强,是无菌性松动深入研究值得推荐的实验方法。Objective To establish the osteolysis animal models induced by titanium particles in murine calvaria. Methods 30 male BALB/c mice were randomly divided into control group and model group, each group was only 15. The mouse calvar- ia was cut open and the periosteum was exposed. 0.3 mL titanium particles mixture was injected into the periosteum defect area of rat in the model group and 0.3 mL sterile fluid of PBS was injected in the control group. The surface of the murine calvaria of the periosteum defect area was observed by macroscopic observation, pathological and tartrate - resisitant acid phosphatase staining examination after fourteen days. Results In macroscopic observation,the surface of the murine calvaria of periosteum defect area was holonomic and had bright luster in the control group. In the model group, the surface of the murine calvaria of periosteum defect area was attacked by titani- um particles and bone absorption. The pathologic results showed that the bone absorption was not significant, the bone trabecula was e- venly distributed, arranged regularly, thick and dense, and no titanium particles have been found in the control group, however, bone ab- sorption was obvious, cell morphology was irregular, bone trabecula was sparsely disturbed, spacing widened and bone trabecula interrup- tion phenomenon was appeared in the model group. The bone resorption area was （0. 098±0. 012） mm2 in the control group, and （0.365±0.113 ）mm2 in the model group, the two groups had significant differences. The TRAP staining results showed that the staining was shallow, the positive staining area was smaller, and the number of cells was not large in the control group and the staining was deep, the positive staining area was larger, the number of cells was increased significantly and there was bone absorption phenomenon in the model group. The number of TRAP - positive cells were （ 11.42±1.21 ） in the control group and （ 27.82±0.24 ） in the model group, there was a significant difference between t

关 键 词：钛颗粒 颅骨 骨溶解 动物模型 

分 类 号：R687[医药卫生—骨科学]