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作 者:彭鑫[1] 王喜乐 倪彬彬 陈思兰 叶超琳 胡君艳[1,2] 左照江[1] PENG Xin;WANG Xile;NI Binbin;CHEN Silan;YE Chaolin;HU Junyan;ZUO Zhaojiang(School of Forestry and Biotechnology,Zhejiang Agriculture & Forestry University,Hangzhou 311300,Zhejiang,China;National Demonstration Center for Experimental Forestry Education,Zhejiang Agriculture & Forestry University,Hangzhou 311300,Zhejiang,China)
机构地区:[1]浙江农林大学林业与生物技术学院,杭州311300 [2]浙江农林大学国家林业本科教学示范中心,杭州311300
出 处:《果树学报》2018年第9期1087-1097,共11页Journal of Fruit Science
基 金:浙江省自然科学基金资助项目(LY17C160004);国家自然科学青年基金资助项目(31300364);浙江农林大学科研发展基金人才启动项目(2013FR069)
摘 要:【目的】研究遮阴处理对草莓叶片光合特性及果实品质的影响。【方法】以草莓‘章姬’品种为试材,测定其叶片光合特性、糖含量及果实糖、可溶性蛋白、花青素、可溶性固形物和总酚含量、可滴定酸度、香气成分。【结果】50%遮阴处理后,草莓叶片叶绿素荧光参数φPO、ΨO、φEO和RC/CSM均较未遮阴处理显著降低(p<0.05),φDO显著升高(p<0.05);而25%遮阴处理与未遮阴处理间无显著差异。蔗糖是光合产物的主要运输形式,25%遮阴处理使其含量在草莓果实中增加了21.17%(p<0.05),在叶片中降低了38.88%(p<0.05),这表明25%遮阴处理可促进蔗糖向果实转运。此外,25%遮阴处理后,果实中可滴定酸度降低了12.84%(p<0.05),其他品质指标与未遮阴处理间无显著差异。50%遮阴处理后,果实中总酚、葡萄糖、还原糖和总糖含量均显著降低(p<0.05)。与未遮阴处理相比,25%和50%遮阴处理后,草莓果实萜烯类香气释放量分别增加了22.53%(p<0.05)和9.84%。【结论】25%遮阴处理可促进光合产物(蔗糖)转运,提高果实蔗糖含量,降低可滴定酸度,提高香气释放。因此,浙江省及其相同气候区春夏季草莓设施栽培适宜进行25%遮阴处理以提高果实品质。【Objective】 Light as the energy source, can affect plant photosynthesis. Both excessive and insufficient light conditions damage photosynthetic structures, and reduce photosynthetic efficiency and eventually fruit quality. Shading treatment is a practical method widely used in facility cultivation for high-quality strawberry, but the suitable shading level is not clear. To uncover the suitable shading level and provide theoretical basis for high-quality strawberry production during spring and summer in Zhejiang and in regions with similar climate, the effects of shading on leaf photosynthetic rate and fruit quality were investigated in this study. 【Methods】 In February,‘Akihime' cultivar seedlings with the same growth status were selected as the experimental materials. Sheds were built and covered with shading net to set 25% and 50% shading treatments in March, and the shed without shading net was used as the control (0% shading treatment). During the strawberry ripening period (approximately 45-60 days after treatment), chlorophyll fluorescence was measured with a Yaxin-1161 chlorophyll fluorescence analyzer, and various fluorescence parameters were calculated. All the leaves in each plant were harvested to measure the sugar content using anthrone colorimetry method, and the sugar content in fruits were determined using the same method. The contents of soluble proteins, total phenols and anthocyanins in fruitwere determined with the coomassie brilliant blue colorimetry method, the Folin-phenol method, and the spectrophotometric method with pelargonidin-3-O-glucoside as the standard, respectively. Titrable acidity was measured with titration method, and soluble solid content with an Abbe refractometer. The aroma compounds were extracted by the solid phase microextraction (SPME) method, analyzed with a gas chromatography-mass spectrometer (GC-MS), and identified according to NIST library. 【Results】 In the treatments with 25% and 50% shading, the maximum quantum yield of primary phot
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