气滞血瘀与气虚血瘀心肌梗死大鼠模型的生物学标记物表达差异  

Difference of Biological Markers Expression between Myocardial Infarction Model Rats with Qi Stagnation and Blood Stasis Type and Qi Deficiency and Blood Stasis Type

作　　者：许颖智[1] 王晓玲 庞树朝[1] 徐士欣[1] 袁卓[1] 张军平[1] XU Yingzhi;WANG Xiaoling;PANG Shuchao;XU Shixin;YUAN Zhuo;ZHANG Junping(The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin,300000;Qian＇an Hospital of Traditional Chinese Medicine)

机构地区：[1]天津中医药大学第一附属医院 [2]迁安市中医院

出　　处：《中医杂志》2018年第18期1606-1610,共5页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(30901890)

摘　　要：目的探讨心肌梗死气虚血瘀和气滞血瘀证动物模型的生物学特点。方法雄性Wistar大鼠36只随机分为正常组6只、心肌梗死组10只、气虚血瘀组10只、气滞血瘀组10只。冠状动脉左前降支结扎造成心肌梗死模型,在此基础上,采用饥饿、疲劳、寒湿刺激建立心肌梗死气虚血瘀证模型,采用昼夜失调、闪烁光刺激和夹尾刺激联合冰水处理建立心肌梗死气滞血瘀证大鼠模型,观察大鼠的一般情况、心肌组织病理染色等情况;检测血小板聚集率、血浆血小板膜蛋白140(GMP-140)、降钙素基因相关肽(CGRP)及去甲肾上腺素(NE)水平。结果气虚血瘀组大鼠倦怠嗜睡,活动量少;气滞血瘀证组大鼠活动量增多,精神亢奋,极易激惹。心肌组织NBT染色显示心肌梗死组、气虚血瘀组和气滞血瘀组大鼠均可见灰白色梗死区域。心肌梗死组、气虚血瘀组及气滞血瘀组在60 s、180 s、300 s及最大值的血小板聚集率均高于正常组(P<0.01),心肌梗死组、气虚血瘀组及气滞血瘀组各时点的血小板聚集率两两比较,差异均无统计学意义(P>0.05)。心肌梗死组、气虚血瘀组及气滞血瘀组GMP-140高于正常组(P<0.05或P<0.01),且气虚血瘀组及气滞血瘀组GMP-140水平均高于心肌梗死组(P<0.05);气虚血瘀组CGRP水平低于其他各组(P<0.05);气滞血瘀组NE水平显著高于其他各组(P<0.01)。结论心肌梗死气滞血瘀证与气虚血瘀证生物学标记物表达存在差异性,气虚血瘀证血浆GMP-140、血小板聚焦率升高,CGRP降低;气滞血瘀组血浆GMP-140、血小板聚焦率及NE升高。Objective To study the biological features of animal model of myocardial infarction with qi deficiency and blood stasis syndrome and qi stagnation and blood stasis syndrome. Methods A total of 36 male Wistar rats were randomly divided into 4 groups： normal group（ n = 6）,myocardial infarction model group（ n = 10）,qi deficiency and blood stasis syndrome（ n = 10） and qi stagnation and blood stasis syndrome（ n = 10）. The models of myocardial infarction was made by ligating the anterior decending branches of the left coronary artery. On this basis,the models of myocardial infarction with qi deficiency and blood stasis syndrome were established by hunger,fatigue,cold and dampness,and the models of myocardial infarction with qi stagnation and blood stasis syndrome were established by circadian disorders,scintillation and clipper stimulation combined with ice water. The genera condition,pathologic staining of myocardium tissues of rats were observed. The platelet aggregation rate,and the levels of GMP-140,CGRP and NE were detected. Results The rats in qi deficiency and blood stasis syndrome group were burnout and lethargy,few of activities. Qi stagnation and blood stasis syndrome group was more excited and irritated,and had more activities. GMP-140,NE of 3 model groups were all higher than that of normal group. CGRP of 3 model groups were all lower than that of normal group,with statistical significance（ P〈0. 01）. GMP-140 of qi deficiency and blood stasis group and qi stagnation and blood group were higher than myocardial infarction model group. CGRP of qi deficiency and blood stasis group was lower than qi stagnation and blood group and myocardial infarction model group. NE of qi stagnation and blood group was higher than that of others（ P〈0. 01）. Infarction region of 3 model groups was pale after NBT staining. Conclusion qi deficiency and blood stasis syndrome and qi stagnation and blood stasis syndrome models established by multifactor combined methods,are combined with myocardial infarct

关 键 词：心肌梗死 气虚血瘀 气滞血瘀 病证结合 动物模型 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]