UPLC-ELSD法同时测定不同产地知母中7个成分的含量  

Simultaneous Determination of Seven Constituents in Anemarrhenae asphodeloides by UPLC-ELSD

作　　者：王雅丽 黄琪 吴德玲 贾鹏晖 金传山 黄晓燕 WANG Yali;HUANG Qi;wu Deling;JIA Penghui;JIN Chuanshan;HUANG Xiaoyan(College of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012 Anhui,China;Anhui Key Laboratory of Modern Chinese Medicine,Hefei 230012 Anhui,China;AnHui Guangyintang Chinese Medicine Company,Bozhou 236800 Anhui,China)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]安徽省现代中药重点实验室,安徽合肥230012 [3]安徽广印堂中药股份有限公司,安徽亳州236800

出　　处：《中药新药与临床药理》2018年第5期637-640,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：安徽高校自然科学重大研究项目(KJ2018ZD030);安徽高校自然科学重点项目(KJ2018A0298);安徽省高校科研创新平台团队建设项目(2015TD035);国家中药标准化建设项目(ZYBZH-Y-AH-02)

摘　　要：目的建立超高效液相色谱-蒸发光散射检测器法(UPLC-ELSD)测定不同产地知母中新芒果苷、芒果苷、知母皂苷B-Ⅱ、知母皂苷B-Ⅲ、宝藿苷Ⅰ、知母皂苷A-Ⅲ、菝葜皂苷元等7个成分的含量。方法采用UPLC-ELSD法,ACQUITY BEH C18色谱柱(2.1 mm×100 mm,1.7μm);流动相为乙腈(A)-0.2%冰醋酸(B),梯度洗脱;蒸发光散射检测器(ELSD)雾化器(氮气)流量为2.5 L·min^(-1);蒸发温度80℃;载气压力3.14 bar;进样体积10μL。结果 7个化合物分离度良好,呈现较好的线性关系(r>0.999 0),平均加样回收率均在96.98%~102.60%,RSD≤2.80%。结果显示7个被测成分在不同产地之间含量差异较大。结论该方法简单准确,省时高效,可为不同产地知母药材质量控制提供科学依据。Objective To establish a UPLC-ELSD method for simultaneous determination of neomangiferin,mangiferin,timosaponin B-Ⅱ,timosaponin B-Ⅲ,baohuoside-I,timosaponin A-Ⅲand sarsasapogenin in Anemarrhena asphodeloides from different producing areas.Methods UHPLC-ELSD analysis was performed on an UltiMate3000 apparatus with an ACQUITY BEH C18 column（2.1 mm×100 mm,1.7μm）,acetonitrile（A）and 0.2%glacial acetic acid（B）were selected as the mobile phase,atomizer（nitrogen）flow rate of evaporative light scattering detector（ELSD）was 2.5 L·min^-1,and the evaporation temperature was 80℃;the gas carrying pressure was 3.14 bar,and the injection volume was 10μL.Results The separation of seven compounds is good with a linear relationship（r〉0.9990）.The average sample recoveries were in the range of 96.98%-102.60%with an RSD2.80%.Conclusion The method is rapid,accurate,with high stability and repeatability,which could provide references for the quality control of Anemarrhenae rhizoma.

关 键 词：知母 超高效液相色谱-蒸发光散射检测器法 含量测定 质量控制 

分 类 号：R284.1[医药卫生—中药学]