RP-HPLC法测定延胡索中黄连碱、去氢延胡索甲素、延胡索乙素的含量  

Simultaneous determination of coptisine, dehydrofusone and tetrahydropalmatine in Corydalis Rhizoma by RP-HPLC

作　　者：王雨笛 杨婧[2] 吴高松[1] 刘华[1] 梁雅琪 王艳艳[3] WANG Yu-dia;YANG Jingb;WU Gao-songb;LIU Huaa;LIANG Ya-qia;WANG Yan-yanc(a.Key Laboratory of Chinese Materia Medica（Ministry of Education）,Heilongjang Key Laboratory of TCM Pharmacodynamic Material Base;b.Department of Pathology,Basic Medical Colleg;c.Department of Pharmacology of Traditional Chinese Medicine,Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学教育部北药基础与应用研究重点实验室/黑龙江省中药天然药物药效物质基础重点实验室,黑龙江哈尔滨150040 [2]黑龙江中医药大学基础医学院病理学研究室,黑龙江哈尔滨150040 [3]黑龙江中医药大学中药药理学研究室,黑龙江哈尔滨150040

出　　处：《化学工程师》2018年第9期20-22,共3页Chemical Engineer

基　　金：国家自然基金青年金项目(No.81603418)

摘　　要：目的:建立反向高效液相(RP-HPLC)色谱法同时测定延胡索中黄连碱、去氢延胡索甲素、延胡索乙素的含量测定方法。方法:采用Agilent HC-C_(18)(200×4.6mm,5μm)色谱柱,流动相为乙腈-0.3%乙酸水溶液(三乙胺调pH值为5.0),梯度洗脱,流速为1mL·min^(-1),检测波长282nm。结果:40min内延胡索药材中黄连碱、去氢延胡索甲素、延胡索乙素3个成分完全分离,峰面积与浓度呈现良好的线性关系(r=0.9995~0.9999);平均加样回收率在95.5%~98.8%,RSD为2.0%~3.0%。测定延胡索中黄连碱、去氢延胡索甲素、延胡索乙素含量分别为1.94%、2.23%、0.53%。结论:该方法快捷可靠,适用于延胡索中黄连碱、去氢延胡索甲素、延胡索乙素3个生物碱类成分的含量测定。Objective：To establish a reversed phase high performance liquid chromatography （RP-HPLC） method for the coptisine, dehydrofusone and tetrahydropalmatine in Corydalis Rhizomavsimuhaneously.Methods：Agilent HC-C18（200×4.6mm, 5μm） column was used. The mobile phase consisted of acetonitrile-0.3% acetic acid in water （triethylamine adjusted to pH5.0） with gradient elution at a flow rate of 1 mL·min-1, detection wavelength 282nm.Results：In 40 min, the three compounds of coptisine, dehydrofusone and tetrahydropalmatine in Corydalis Rhizoma were completely separated The peak area and concentration showed a good linear relationship （r =0.9995～0.9999）. The average recovery rate was 95.5.% ～98.8%, the RSD was 2.0% - 3.0%. The determination of coptisine, dehydrofusone and tetrahydropalmatine in Corydalis Rhizoma were 1.94% ,2.23% ,0.53%respectively.Conclusion：This method is rapid and reliable. It is applicable to the determination the three alkaloids of coptisine, dehydrofusone and tetrahydropalmatine in Corydalis Rhizoma.

关 键 词：延胡索 HPLC 含量测定 黄连碱 去氢延胡索甲素 延胡索乙素 

分 类 号：R284[医药卫生—中药学]