血液肿瘤患者血浆EB病毒和巨细胞病毒定量检测的临床价值  

Clinical value of quantitative detection of plasma EB virus and cytomegalovirus in patients with hematological malignancies

作　　者：王海滨[1] 张冬青[1] 司英丽 赵娇[1] WANG Haibin;ZHANG Dongqing;SI Yingli;ZHAO Jiao(Department of Clinical Laboratory,First Affiliated Hospital of PLA General Hospital,Beijing 100048,China)

机构地区：[1]中国人民解放军总医院第一附属医院检验科,北京100048

出　　处：《检验医学与临床》2018年第19期2852-2854,2858,共4页Laboratory Medicine and Clinic

基　　金：国家重大科学仪器设备开发专项基金资助项目(2013YQ1904670703);解放军总医院临床科研扶持基金资助项目(三〇四专项:2015FC-TSYS-3047)

摘　　要：目的采用实时荧光定量聚合酶链反应(PCR)测定血液肿瘤患者血浆中EB病毒(EBV)和巨细胞病毒(CMV)DNA水平,探讨血浆EBV DNA和CMV DNA定量测定的临床意义。方法采用实时荧光定量PCR检测280例血液肿瘤患者血液中EBV DNA和CMV DNA载量。结果 280例血液患者中EBV DNA检测阳性率为17.1%,CMV检测阳性率为18.9%,动态监测病毒DNA拷贝数为10~2~10~7 copy/mL。接受外周造血干细胞移植的患者126例,其中EBV检测阳性率为28.6%,CMV检测阳性率为40.5%。血液肿瘤患者进行抗病毒治疗后,均在7~14d后转阴。结论实时荧光定量PCR动态检测血液肿瘤患者EBV DNA和CMV DNA水平对于EBV和CMV感染患者的疗效判断具有重要临床意义。Objective To adopt real time fluorescent quantitative PCR assay to detect plasma EB virus （EBV） and cytomegalovirus （CMV） DNA levels in the patients with hematological malignancies and to investigate their clinical significance. Methods The real time fluorescent quantitative PCR assay was adopted to detect plasma EBV and CMV DNA loads in 280 patients with hematological malignancies. Results Among 280 cases of hematological malignancies,the EBV detection positive rate was 17.1% and the CMV detection positive rate was 18.9%.The viral DNA copy number by dynamic monitoring ranged 10 2-10 7 copy/mL.Among 126 patients receiving peripheral hematopoietic stem cell transplantation （HSCT）,the EBV detection positive rate was 28.6% and the CMV detection positive rate was 40.5%.After antiviral therapy in the hematological patients,the negative-conversion was obtained after 7-14 d. Conclusion Adopting the real time fluorescent quantitative PCR assay for detecting EBV and CMV DNA level has an important clinical significance for judging the curative effect in the patients with EBV and CMV infection.

关 键 词：EB病毒 巨细胞病毒 血液肿瘤 

分 类 号：R373.9[医药卫生—病原生物学]