篮子鱼属线粒体基因组序列系统发育信息分析  

Phylogenetic information analysis of mitochondrial genome sequences in Siganus(Perciformes: Siganidae)

作　　者：黄小林 杨育凯[1,2] 林黑着 李涛[1,2] 虞为 黄忠[1,2] HUANG Xiao-lin;YANG Yu-kai;LIN Hei-zhao;LI Tao;YU Wei;HUANG Zhong(Shenzhen Base of South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shenzhen 518121;Key Laboratory of South China Sea Fishery Research Exploitation ＆ Utilization,Ministry of Agriculture,South China Sea Fisheries Research Institute,Chinese Academy ofFishery Sciences,Guangzhou 510300,China)

机构地区：[1]中国水产科学研究院南海水产研究所深圳试验基地,深圳518121 [2]中国水产科学研究院南海水产研究所农业部南海渔业资源开发利用重点实验室,广州510300

出　　处：《生物学杂志》2018年第5期33-36,共4页Journal of Biology

基　　金：深圳市科技计划知识创新基础研究项目(JCYJ20170817103922921;JCYJ20170817103856495);广东省渔业生态环境重点实验室开放基金(FEEL-2017-13);广东省渔港建设与渔业发展专项海洋渔业科技推广方向-科技攻关与研发项目(A201601B10)

摘　　要：分析了6条篮子鱼的mtDNA全序列,揭示了篮子鱼mtDNA的基本结构、变异位点、基因选择压力及系统进化等信息。结果表明:1)篮子鱼mtDNA由37个基因组成,其中ND3基因的变异位点比例最高(22. 77%),比例最小为12S rRNA(4. 01%); 2) mtDNA蛋白编码基因(13个)的Ka/Ks均远小于1,且Tajima's D=0. 123 74~0. 615 14(P> 0. 10)均不显著大于或小于0,表明篮子鱼mtDNA蛋白编码基因进化过程中均受到的稳定性(负)选择作用较强; 3)系统进化分析表明,尖嘴篮子鱼与狐篮子鱼最先聚成一支,再依次与眼带篮子鱼和点篮子鱼聚类,之后与褐篮子鱼和长鳍篮子鱼聚成的一支聚类,这种进化顺序与各篮子鱼体色变化一致; 4) COX1、ND4和Cytb基因是篮子鱼属内种间系统进化的理想分子标记。mtDNA complete sequences of 6 Siganus were analyzed, and the basic structure, variation site, gene selection pressure and phylogenetic evolution of mtDNA in Siganus were revealed. mtDNA in Siganus was composed of 37 genes, among which, the highest proportion of variation site was ND3 gene （22.77%）, and the minimum proportion was 12S rRNA （4.01%）. The mtDNA protein coding genes （13）of Ka/Ks were far less than 1, and Tajima′s D = 0.123 74 ～ 0.615 14 （ P 〉0.10）were not significantly greater than or less than 0, which showed that the stability （negative）selection of mtDNA protein coding genes in Siganus was stronger during its evolutionary process. Phylogenetic analysis showed that S. unimaculatus and S. vulpinus were firstly clustered into one group, and then in turn clustered with S. puellus and S. guttatus , finally clustered into one group with S. fuscescens and S. canaliculatus . This evolutionary order is consistent with changes in body color of Siganus . COX1, ND4 and Cytb genes are ideal molecular markers for phylogenetic evolution in Siganus .

关 键 词：篮子鱼属 线粒体 系统发育 分子标记 

分 类 号：Q951.3[生物学—动物学]