杜仲内生细菌DZSY21诱导玉米抗小斑病的系统抗性研究  

Research on maize system resistance triggered by the endophytic bacterium DZSY21

作　　者：孙黄兵 钟年孝 孔令茹 丁婷[1] 马元山 SUN Huang-bing;ZHONG Nian-xiao;KONG Ling-ru;DING Ting;MA Yuan-shan(School of Plant Protection;The Management Center of Agricultural Garden,Anhui Agricultural University,Hefei 230036,China)

机构地区：[1]安徽农业大学植物保护学院,合肥230036 [2]安徽农业大学农业园管理中心,合肥230036

出　　处：《生物学杂志》2018年第5期49-53,共5页Journal of Biology

基　　金：国家自然科学基金面上项目(31371980);安徽省大学生创新创业训练计划项目(201610364082)

摘　　要：前期试验表明杜仲内生细菌DZSY21可在玉米中定殖并显著增强玉米对小斑病的抗病性。在此基础上,研究了杜仲内生细菌DZSY21对玉米的防御相关酶活性,抗病信号传导通路的标志基因表达的诱导情况。结果显示杜仲内生细菌DZSY21引入玉米植株,在3～10 d内玉米叶片中PPO、POD和PAL等防御酶的活性都有不同程度的持续增加,与对照相比差异显著;此外,玉米水杨酸(SA)信号通路激发的防卫基因PR-1和茉莉酸(JA)信号通路激发的防卫基因LOX,分别在24～60 h以及12～60 h得到了显著持续高表达。以上结果表明,利用杜仲内生细菌DZSY21处理玉米后,能够通过增强植物防御酶活性,诱导植株形成水杨酸(SA)和茉莉酸(JA)信号传导通路,来提高玉米植株抗病能力。Preliminary tests showed the endophytic bacterium DZSY21 isolated from Eucommia ulmoides could colonize in maize, and the resistance of maize to Bipolaris maydis was enhanced by colonization of DZSY21. In this study, the resistance-related enzymes activity and signal transduction pathways were investigated to clarify the mechanism of maize systemic resistance triggered by the endophytic bacterium DZSY21. After leaves treated with DZSY21, the activities of PPO, POD and PAL of the maize leaves increased at 3-10 days. Meanwhile, the defense gene PR-1 in salicylic acid（SA） signaling pathway and the gene LOX in jasmonic acid （JA） signaling pathway were expressed continuously at 24-60 h and 12-60 h, respectively. Accordingly, the DZSY21 was capable of inducing systemic resistance in maize plants. In summary, the application of the endophytic bacterium DZSY21 could trigger maize systemic disease resistance which plays an important role in the control of Bipolaris maydis.

关 键 词：内生细菌 玉米 防御酶 信号传导通路 

分 类 号：S513[农业科学—作物学] Q943.2[生物学—植物学]