DNA甲基化测序技术及其在哺乳动物中的应用研究进展  

Research progress of DNA methylation sequencing technology and application in mammal

作　　者：段昕妤 肖蘅[1] 陈善元[1] DUAN Xin-yu;XIAO Heng;CHEN Shan-yuan(School of Life Sciences,Yunnan University,Kunming 650091,China)

机构地区：[1]云南大学生命科学学院,昆明650091

出　　处：《生物学杂志》2018年第5期79-82,86,共5页Journal of Biology

基　　金：国家自然科学基金(31460281)

摘　　要：DNA甲基化在很多真核生物中是一种非常重要的表观遗传学标记,结合新一代测序技术(next-generation sequencing,NGS)对哺乳动物的DNA甲基化水平进行定量测定已成为目前的研究热点。具有高通量、高覆盖率、高分辨率等优点,能较为精准地检测全基因组范围内的甲基化位点。对限制性内切酶消化法、亲和富集法及重亚硫酸盐转化法进行归纳比较,以期为DNA甲基化测序技术的选择提供参考依据,并对测序技术进行展望。DNA methylation is an important epigenetic mark in many eukaryotes. The quantitative measurement of mammal′s genome-wide DNA methylation level has become a research hotspot by combining with the next-generation sequencing （NGS） technologies. With the advantages of high-throughput, high coverage and high resolution through the comparison with other sequencings, NGS technologies provide an important approach for accurately detecting DNA methylation sites within the whole genome. In order to provide a reference for the selection of DNA methylation sequencing technology, three methods of restriction endonuclease digestion, affinity enrichment and bisulfite conversion were summarized and compared in this paper, and the future development of sequencing technology was predicted.

关 键 词：哺乳动物 DNA甲基化 限制性内切酶消化法 亲和富集法 重亚硫酸盐转化法 

分 类 号：Q31[生物学—遗传学]