多重PCR技术在转基因成分检测中的最新研究进展  

Review on multiplex polymerase chain reaction for the detection of genetically modified components

作　　者：鲁军 李刚[1] 赵建宁[1] 杨殿林[1] 修伟明[1,2] LU Jun;LI Gang;ZHAO Jian-ning;YANG Dian-lin;XIU Wei-ming(Ecological Environment Safety Supervision,Inspection and Testing Center of GMOs of Ministryof Agriculture/Agro-Environmental Protection Institute,Ministry of Agriculture,Tianjin 300191;College of Plant Protection,Shenyang Agriculture University,Shenyang 110866,China)

机构地区：[1]农业部环境保护科研监测所农业部转基因生物生态环境安全监督检验测试中心,天津300191 [2]沈阳农业大学植物保护学院,沈阳110866

出　　处：《生物学杂志》2018年第5期87-90,共4页Journal of Biology

基　　金：中央级公益性科研院所基本科研业务费专项资金(农业部环境保护科研监测所);国家自然科学基金青年科学基金项目(31200424)

摘　　要：随着全球转基因作物种植面积的迅速增加和商品化进程的不断加快,转基因植物及其产品的安全性引发人们的广泛关注。世界许多国家和地区不断加大对转基因植物及其产品的监管力度,而精准、高效、低成本的转基因成分检测技术无疑成为转基因产品监管和标识的重要技术支撑。多重PCR(Multiplex polymerase chain reaction,Multiplex PCR)技术由于其具有高通量、特异性好、简单高效、节省实验样品和试剂等优点,得到了众多科研工作者的青睐并迅速在各行业中得到推广。综述了Multiplex PCR技术在转基因成分不同检测需求方面的应用以及与其他技术相结合的最新研究进展,提出了持续改进的策略。As the planting area of the global genetically modified crops has bear increasing rapidly and the commercialized process is accelerated, people have paid more attention to the safety of genetically modified plants and derived products. The supervision of genetically modified plants and derived products has been paid more attention by many countries and areas. The accurate, efficient, and inexpensive detection methods of genetically modified components are the important technical support for supervision and labeling of genetically modified food and feed. Multiplex polymerase chain reaction （Multiple PCR） technology has captured the attention of more and more researchers and has been popularized and applicated in many kinds of industries because of its low cost, high flux, better specificity, saving the experimental samples and medicine. This paper showes the latest research progress on multiple PCR detection method combined with other technology and the application to different detect requirement of genetically modified ingredients. The paper also gives further strategies for improving Multiple PCR detection methods of genetically modified components.

关 键 词：多重PCR 转基因成分 检测 

分 类 号：G943.2[文化科学]