泡桐组培中的脱菌技术  

Virus-free in Tissue Culture of Paulownia

作　　者：武文豪 李小凡 翟晓巧[2] Wu Wenhao;Li Xiaofan;Zhai Xiaoqiao(Institute of Paulownia,Henan Agrieultural University,Zhengzhou 450002,China;Henan Academy of Forestry,Zhengzhou 450008,China)

机构地区：[1]河南农业大学泡桐研究所,河南郑州450002 [2]河南省林业科学研究院,河南郑州450008

出　　处：《河南林业科技》2018年第3期1-4,共4页Journal of Henan Forestry Science and Technology

基　　金：河南省科技创新杰出创新人才计划项目;项目编号:174200510001

摘　　要：以细菌污染的白花泡桐组培苗为材料,采用70%的酒精,0.1%HgCl_2和不同时间梯度的70%的酒精结合0.1%HgCl_23种方法来研究泡桐组培苗的脱毒技术,结果显示70%的酒精(10s)结合0.1%HgCl_2(7min)的方法效果最好,外植体的脱菌率为65.0%。In this study, the bacterial-contaminated paulownia fortuneitissue culture seedlings were used as materials, Using 70% alcohol, 0.1% HgCl2 and 70% alcohol with different time gradient combined with 0.1% HgCl2 3 method to study the virus-free technology of Paulownia tissue culture seedlings, The results showed that 70% alcohol （ 10s ） combined with 0.1% HgCl2 （ 7 min ） was the best, and the explant removal rate was 65.0%.

关 键 词：白花泡桐 细菌污染 脱毒 

分 类 号：S792.43[农业科学—林木遗传育种]