猪流行性腹泻病毒CZ2014株的分离鉴定  

Isolation and identification of porcine epidemic diarrhea virus CZ2014

作　　者：李松玲 郑素雅 周红[1] 范红结[1] 蔺辉星[1] LI Songling;ZHENG Suya;ZHOU Hong;FAN Hongjie;LIN Huixing(College of Veterinary Medicine,Nanjing Agricultural University/MOE Joint International Research Laboratory of Animal Health and Food Safety/Key Lab of Animal Bacteriology,Ministry of Agriculture,Nanjing 210095,China)

机构地区：[1]南京农业大学动物医学院/教育部动物健康与食品安全国际合作联合实验室/农业部动物细菌学重点实验室

出　　处：《畜牧与兽医》2018年第9期46-50,共5页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金青年科学基金(31702278);江苏省博士后科研资助计划(1501030C)

摘　　要：2014年江苏某规模猪场发生腹泻疫情,采集发病仔猪小肠组织及肠内容物,经RT-PCR检测,仅猪流行性腹泻病毒（PEDV）阳性。将病料接种Vero细胞,盲传3代后,出现明显的细胞合胞体病变,细胞空泡化,最终裂解脱落。将细胞反复冻融后,收获病毒,经RT-PCR以及间接免疫荧光（IFA）鉴定后,确认所收获的病毒为PEDV,将该分离毒株命名为CZ2014株（Gen Bank：KT428878.1）。RT-PCR扩增其S基因,序列分析结果表明,CZ2014株S基因序列与近年来流行的毒株同源性较高,而与经典疫苗毒株同源性较低。PEDV流行毒株的分离为该病毒致病机制研究及其防控奠定了基础。In 2014,diarrhea epidemic occurred on a scale pig farm in Jiangsu Province. Samples of the small intestine mucosa and intestinal contents were collected and examined by RT-PCR,and they were PEDV positive. The samples were then inoculated onto Vero cells with10 μg/m L trypsin,and the PEDV CZ2014 strain（Gen Bank： KT428878. 1） was isolated. Phylogenetic analysis was performed,showing that the CZ2014 strain was of low homology to the traditional vaccine strain located in different branches,but more homologous to the epidemic strains isolated in recent years. The present results indicated that the YC2014 strain might be a candidate vaccine strain for PEDV immune control.

关 键 词：猪流行性腹泻病毒 流行毒株 分离 

分 类 号：S852.6[农业科学—基础兽医学]