不同试剂盒检测接种FMDV的牛非结构蛋白抗体结果及分析  

Analysis of the results of non-structural protein antibody in cattle inoculated with FMDV using different kits

作　　者：王文莉[1,2] 赵俊皓 刘华南 卢炳州[2] 魏南南 张学刚 郑海学 胡永浩[1] 张克山[2] WANG Wenli;ZHAO Junhao;LIU Huanan;LU Binzhou;WEI Nannan;ZHANG Xuegangz;ZHENG Haixue;HU Yonghao;ZHANG Keshan(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730046,China;Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences/State Key Laboratory of Veterinary Etiological Biology/Key Open Laboratory of Animal Virology,Ministry of Agriculture/National Foot and Mouth Disease Reference Laboratory,Lanzhou 730046,China)

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院兰州兽医研究所/家畜疫病病原生物学国家重点实验室/农业部畜禽病毒学重点开放实验室/国家口蹄疫参考实验室.甘肃兰州730046

出　　处：《畜牧与兽医》2018年第9期56-62,共7页Animal Husbandry & Veterinary Medicine

基　　金：国家科技支撑计划(2015BAD12B04);国家自然科学基金-联合基金(U1501213)

摘　　要：选取国内外3家不同的研发公司的甲、乙、丙3种试剂盒,利用ELISA方法分别检测了接种A型与O型口蹄疫病毒的牛口蹄疫病毒（FMDV）非结构蛋白3ABC抗体的变化规律。结果：接种A型与O型口蹄疫病毒的牛在感染后第3天发病,这与最早检测出阳性的时间相一致,至第9天3ABC抗体全部为阳性;接种A型口蹄疫病毒的牛与接种O型口蹄疫病毒的牛3ABC非结构蛋白抗体应答有差异。选取的3种试剂盒检测显示,每2种试剂盒呈现不同程度的相关性,并且甲试剂盒显示出最大的灵敏度,而乙试剂盒显示最大的特异性,丙试剂盒的敏感性依可疑样品的处理方式而变化。因此,对3种试剂盒进行比较发现甲试剂盒更稳定,更适合于检测。In this study,three different ELISA kits were used to detect the changes in 3 ABC antibody of FMDV non-structural protein in cattle inoculated with type A and type O foot-and-mouth disease viruses（FMDV）. The results showed that cows inoculated with both type A and type O foot-and-mouth disease virus showed clincal signs on the third day after infection,which was consistent with the earliest positive detection time. All antibodies against 3 ABC were positive on the 9 th day; but the 3 ABC non-structural protein antibody response in the cattle inoculated with type A FMDV was different from that in the cattle inoculated with type O-type FMDV. The three kits we used in the experiment showed different degrees of correlation between every two,and Kit A showed the highest sensitivity,while Kit B showed the highest specificity,and the sensitivity of Kit C depended on the process of suspected samples. As a result,Kit A was more stable and more suitable for detection here.

关 键 词：口蹄疫病毒 非结构蛋白 3ABC抗体 

分 类 号：S854.43[农业科学—临床兽医学]