UPLC-Q-TOF-MS分析补肾强身片中化学成分  

Analysis on Chemical Constituents in Bushen Qiangshen Pills by UPLC-Q-TOF-MS

作　　者：林秀莲 宋粉云 潘玄玄 谢淑桐 李华[3] LIN Xiu-lian;SONG Fen-yun;PAN Xuan-xuan;XIE Shu-tong;LI Hua(School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China;School of Pharmacy,Guangdong Pharmaceutical University,Guangzhou 510006,China;Guangdong Institute for Drug Control,Guangzhou 510180,China)

机构地区：[1]广东药科大学中药学院,广州510006 [2]广东药科大学药学院,广州510006 [3]广东省药品检验所,广州510180

出　　处：《中国实验方剂学杂志》2018年第19期61-67,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：上海市药物(中药)代谢产物研究重点实验室开放基金项目(2015SHDX1001)

摘　　要：目的：采用超高效液相色谱-四级杆-飞行时间质谱（UPLC-Q-TOF-MS）对补肾强身片中的化学成分进行研究。方法：以Agilent SB-C18色谱柱（4.6 mm×100 mm,2.7μm）,流动相乙腈-0.1%甲酸水溶液,梯度洗脱,流速0.5 m L·min-1,柱温40℃,电喷雾离子源,正、负离子模式下采集数据。首先对有对照品的成分进行定性分析,对于多数没有对照品的成分,主要根据UPLC-Q-TOF-MS提供的准确相对分子质量和离子碎片信息并结合文献数据比对进行化学成分结构推测鉴定。结果：共鉴定了补肾强身片中50个化学成分,包括酚酸类19个、黄酮类25个、裂环环烯醚萜类4个、苯乙醇苷类1个、生物碱类1个,其中12个成分（原儿茶酸,红景天苷,原儿茶醛,绿原酸,金丝桃苷,异槲皮苷,特女贞苷,朝藿定C,淫羊藿苷,山柰酚,宝霍苷I,紫云英苷）通过与对照品比对进行定性分析。结论：UPLC-Q-TOF-MS可以简便、快速地对补肾强身片中化学成分定性分析,该研究丰富了补肾强身片中化学成分的信息,为补肾强身片药效物质基础研究、质量控制及其临床用药提供了参考。Objective： To investigate the chemical constituents in Bushen Qiangshen pills（ BQP） by ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry（ UPLC-Q-TOF-MS）.Method： The UPLC separation was performed on Agilent SB-C18 reversed-phase column（ 4. 6 mm × 100 mm,2. 7 μm） with acetonitrile-0. 1% formic acid solution as mobile phase for gradient elution. The flow rate was0. 5 m L·min-1,and the column temperature was 40 ℃. Data were collected via positive and negative ion mode using ESI ion source. First of all,the qualitative analysis was conducted for the components with reference substances,and the major components without reference substances were identified according to the relative molecule weight and fragment information by UPLC-Q-TOF-MS as well as comparison with literature data. Result：Fifty compounds from BQP were identified, including 19 phenolic acids, 25 flavonoids, 4 olefins, 1 phenylethanolate and 1 alkaloid. 12 components（ protocatechuic acid, salidroside, protocatechuic aldehyde,chlorogenic acid, hyperin, isoquercitrin, specnuezhenide, epimedin C, icariin, kaempferide,baohuoside Ⅰand astragalin） among them were qualitatively analyzed by comparing with reference substances. Conclusion：UPLC-Q-TOF-MS is convenient and rapid to analyze the chemical constituents in BQP,providing a reference for the study on material foundation of efficacy,quality control and clinical application of BQP.

关 键 词：补肾强身片 超高效液相色谱-四级杆-飞行时间质谱 酚酸 黄酮 

分 类 号：R284.1[医药卫生—中药学] R289[医药卫生—中医学]