马铃薯斑纹片病菌检测技术研究进展  

Review of advances in detection of Candidatus Liberibacter solanacearum

作　　者：丁钿 魏霜[2] 李新浩 章柱 易建平[3] 赵文军[4] Drag Tian;wei Shuang;Li Xinhaol;Zhang Zhu;Yi Jianping;Zhao Wenjun(Guangzhou Airport Entry-Exit Inspection and Quarantine Bureau,Guangzhou 510410,China;Guangdong Inspection and Quarantine Technology Center;Shanghai Entry-Exit Inspection and Quarantine Bureau;Chinese Academy of Inspection and Quarantine)

机构地区：[1]广州机场出入境检验检疫局,广东广州510410 [2]广东出入境检验检疫局检验检疫技术中心 [3]上海出入境检验检疫局 [4]中国检验检疫科学研究院

出　　处：《植物检疫》2018年第5期1-7,共7页Plant Quarantine

基　　金：国家重点研发计划项目(2018YFF0214902);广东出入境检验检疫局科技计划项目(2018GDK26)

摘　　要：马铃薯斑纹片病是茄科、伞形科作物一种重要病害,其病原为Candidatus Liberibacter solanacearum,快速、灵敏、特异的检测技术对该病害的发现、预警和防治都具有重要意义。本文综述了国外关于马铃薯斑纹片病菌检测方法的研究进展,重点介绍了以PCR、实时荧光PCR、多重PCR、LAMP等为代表的分子生物学检测技术在马铃薯斑纹片病菌检测中的应用,并分析了各种技术的优缺点,为该病原菌的检测及防控技术研究提供参考。Potato zebra chip disease caused by Candidatus Liberibacter solanacearum（Lso） seriously infects crops of Solanaceae and Umbelliferae. Fast,accurate,sensitive and specific detection methods are very important for surveillance and control of this disease. In this paper,recent advances in molecular detection techniques of Lso were reviewed. The published molecular detection techniques,such as conventional PCR,real-time quantitative PCR,multiplex PCR and LAMP were discussed and the merit and demerit of them were evaluated so as to provide useful reference for the detection and control of Lso.

关 键 词：马铃薯斑纹片病 Candidatus Liberibacter SOLANACEARUM PCR 检测 

分 类 号：S41[农业科学—植物保护]