应用分子生物学方法鉴定南方红豆杉  

Identification of Taxus wallichiana var. mairei with molecular method

作　　者：张明哲 尹文秀 陈哲 王沛[2] 吴姗 许瑾[3] 虞惠贞 张晓峰 李明福[3] Zhang Mingzhe;Yin Wenxiu;Chen Zhe;Wang Pei;Wu Shan;Xu Jin;yu Huizhen;Zhang Xiaofeng;Li Mingfu(Zhejiang Academy of Science and Technology for Inspection and Quarantine,Hangzhou 310016,China;Fujian Entry-Exit In-spection and Quarantine Bureau Technical Center;Chinese Academy of Inspection and Quarantine)

机构地区：[1]浙江省检验检疫科学技术研究院,浙江杭州310016 [2]福建出入境检验检疫局检验检疫技术中心 [3]中国检验检疫科学研究院

出　　处：《植物检疫》2018年第5期39-42,共4页Plant Quarantine

基　　金：国家重点研发计划课题(2017YFF0210304); 浙江省重点研发计划项目(2018C02041); 浙江省科技厅公益性项目(2017C32045)

摘　　要：本研究对南方红豆杉(Taxus wallichiana var.mairei)、东北红豆杉(Taxus cuspidata)、曼地亚红豆杉、欧洲红豆杉(Taxus baccata)、密叶红豆杉(Taxus fuana)、喜马拉雅红豆杉(Taxus wallichiana)和红豆杉(Taxus wallichiana var.chinensis)7种红豆杉属植物的ITS序列进行PCR扩增和测序,并通过DNAMAN软件筛选出多态性位点,设计出南方红豆杉特异性引物和探针。实时荧光定量PCR实验表明,该引物探针能特异性扩增南方红豆杉和喜马拉雅红豆杉,且检测灵敏度可以达到0.001 ng/μL,是一种快速、灵敏的鉴定方法。In this study,the ITS gene of 7 Taxus species（Taxus wallichiana var. mairei,Taxus cuspidata,Taxus ×Media,Taxus baccata,Taxus fuana,Taxus wallichiana and Taxus wallichiana var. chinensis）were amplified and sequenced. Polymorphic loci were screened by DNAMAN software and specific primers and probes were designed for Taxus wallichiana var. mairei. The Real-time PCR test showed that the primer and probe can specifically amplify Taxus wallichiana var. mairei and Taxus wallichiana,and the sensitivity can reach 0.001 ng/μL. It is a sensitive method to identify Taxus wallichiana var. mairei rapidly.

关 键 词：南方红豆杉 实时荧光PCR ITS 物种鉴定 

分 类 号：S791.49[农业科学—林木遗传育种]