温经止痛方对子宫内膜异位症模型大鼠自嗜基因Beclin-1、LC3表达的影响  

Effects of Wenjing Zhitong Recipe on Expression of Autophagy Genes Beclin-1 and LC3 in Endometriosis Rats

作　　者：黄艳辉[1] 丑丹[1] 冯丹[1] 徐琳[1] 刘佳[1] 刘亚玲[1] HUANG Yan-Hui;CHOU Dan;FENG Dan;XU Lin;LIU Jia;LIU Ya-Ling(Dept.of Gynecology,Wuhan Hospital of Traditional Chinese Medicine,Wuhan 430010 Hubei,China)

机构地区：[1]武汉市中医医院妇科,湖北武汉430010

出　　处：《广州中医药大学学报》2018年第5期848-854,共7页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：湖北省自然科学基金资助项目(编号:2015CFB590);武汉市科技局资助项目(编号:2015061701011633);武汉市卫生计生委员会课题(编号:wz15b11);湖北省卫计委2016~2017年度中医药科研项目

摘　　要：【目的】探讨温经止痛方对子宫内膜异位症(EMS)模型大鼠子宫在位/异位内膜自噬基因Beclin-1、LC3表达的影响。【方法】将90只大鼠随机分为假手术组,模型组,中药高、中、低剂量组,西药组等6组,每组15只。中药高、中、低剂量组大鼠给予温经止痛方(剂量分别为74、37、18.5 g·kg^(-1)·d^(-1))灌胃;西药组大鼠给予孕三烯酮胶囊(剂量为0.5 mg·kg^(-1)·d^(-1))灌胃,每周2次;模型组及假手术组大鼠给予等量生理盐水灌胃。均给药8周。采用苏木素—伊红(HE)染色观察子宫内膜病理学变化,分别采用实时定量PCR(qPCR)法、免疫组化法检测在位、异位内膜中Beclin-1、LC3 m RNA及蛋白的表达。【结果】给药后中药高剂量组及西药组异位病灶较给药前缩小(P<0.05)。模型组在位、异位内膜上Beclin-1、LC3 mRNA及蛋白表达水平低于假手术组(P<0.01)。中药高剂量组及西药组的在位、异位内膜上的LC3及Beclin-1 m RNA及蛋白表达水平高于模型组(P<0.01)。【结论】EMS的发生可能与Beclin-1、LC3表达失调有关。温经止痛方可有效缩小异位灶,调整Beclin-1、LC3的表达,抑制异位内膜生长。Objective To observe the effects of Wenjing Zhitong Recipe（WZR）on the expression of autophagy genes Beclin-1 and LC3 in eutopic and ectopic endometrium of endometriosis rats. Methods Ninety rats were randomly divided into 6 groups,namely sham operation group,model group,high-,middle-and low-dose Chinese medicine groups,western medicine group,15 rats in each group. The high-,middle-and low-dose Chinese medicine groups were given intragastric administration of WZR（at the dosage of 74,37,18.5 g·kg^-1·d^-1,respectively）, the western medicine group was given intragastric administration of gestrinone capsules（at the dosage of 0.5 mg · k^-1·d^-1）, and the model group and the sham operation group were given intragastric administration of the same volume of normal saline,the treatment lasting for 8 weeks. The pathologic features of endometrium were observed by haematoxylin-eosin（HE）staining, and the m RNA and protein expression of autophagy genes Beclin-1 and LC3 in eutopic and ectopic endometrium were detected by real-time quantitative PCR（q PCR）and immunohistochemistry, respectively. Results The size of the heterotopic focus in high-dose Chinese medicine group and western medicine group was smaller after treatment than that before treatment（P〈0.05）. Compared with the sham operation group,the m RNA and protein expression levels of Beclin-1,LC3 in model control group were decreased（P〈0.01）. The m RNA and protein expression levels of Beclin-1,LC3 in eutopic and ectopic endometrium of the high-dose Chinese medicine group and the western medicine group were higher than those in the model group（P〈0.01）. Conclusion Endometriosis may be associated with disordered expression of Beclin-1 and LC3. WZR can reduce the ectopic focus effectively,regulate the expression of Beclin-1 and LC3,and inhibit the growth of ectopic endometrium.

关 键 词：温经止痛方 子宫内膜异位症 自噬 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]