新古尼异虫草内菌核的多菌定殖现象  

Multiple fungi colonized in the endo-sclerotia of Metacordyceps neogunnii

作　　者：胡胜兰 梁建东[2] 陈万浩[2] 邹晓[1] 韩燕峰[1] 梁宗琦[1] HU Sheng-Lan;LIANG Jian-Dong;CHEN Wan-Hao;ZOU Xiao;HAN Yan-Feng;LIANG Zong-Qi(lnstitute of Fungus Resources,Department of Ecology,College of Life Sciences,Guizhou University,Guiyang,Guizhou 550025,China;Department of Microbiology,Guiyang College of Traditional Chinese Medicine,Guiyang,Guizhau 550025,China)

机构地区：[1]贵州大学生命科学学院生态系真菌资源研究所,贵州贵阳550025 [2]贵阳中医学院微生物教研室,贵州贵阳550025

出　　处：《菌物学报》2018年第9期1199-1206,共8页Mycosystema

基　　金：国家自然科学基金(31460010);科技部基础工作专项(2013FY110400);贵州省千层次人才项目(2014);贵州省重大科技专项(黔科合重大专项字[2016]3022-7);贵州省生物学一流学科建设项目(GNYL[2017]009)~~

摘　　要：基于高通量测序技术,对采自中国江西省宜丰县杨柳镇、贵州省石阡县白沙镇、贵州省湄潭县、贵州省凤岗县和贵州省施秉县5个不同地区的新古尼异虫草Metacordyceps neogunnii的真菌生态位进行研究。结果显示,新古尼异虫草生境土壤、菌表及其内菌核的真菌类群共涉及子囊菌门Ascomycota、担子菌门Basidiomycota、接合菌门Zygomycota、类原生动物门Rozellomycota 4个门及一些未定门的真菌;共17纲、43目、77科、129个属的177种真菌。在属的水平上,新古尼异虫草生境土壤(Cg1)的优势属为被孢霉Mortierella,相对丰度为84.86%,其次是分类地位未定的真菌(4.58%),毛壳菌科Chaetomiaceae 1属(1.22%);虫草菌表(Cg2)的优势属或种为新古尼异虫草M.neogunnii(83.66%),其次是粉红螺旋聚孢霉Clonostachys rosea(9.48%),帚枝霉属Sarocladium(2.07%)和被孢霉属(1.12%)。而来自江西(JX)、石阡(SX)、凤岗(FG)、施秉(Cg3)4个不同地区的新古尼异虫草样本的内菌核优势属真菌均为新古尼异虫草,其相对丰度分别为:99.74%、99.50%、99.73%和98.82%,其余属相对丰度均小于1%。来自湄潭的干标本内菌核优势属为麦角菌科Clavicipitaceae的一个未定属,相对丰度则高达99.97%。综上结果表明,来自5个不同地区的新古尼异虫草种群的内菌核中不仅是新古尼异虫草M.neogunnii,同时有其他真菌存在。施秉样本周围的土壤真菌群落较虫草样本内菌核具明显的多样性。但有趣的是,采集虫草的土壤中不含新古尼异虫草,而具有相对丰度较大的被孢霉Mortierella spp.和木霉Trichoderma spp.。来自湄潭的干标本,不含新古尼异虫草而是相对丰度较高的麦角菌科Clavicipitaceae一未知成员,其分类地位值得进一步研究。Based on high-throughput sequencing technology, the fungal ecological niche of Metacordyceps neogunnii from five different regions, Yifeng（Jiangxi Province）, Shiqian, Meitan, Fenggang and Shibing（Guizhou Province）, was investigated. The results showed that the fungi from Metacordyceps neogunnii habitat soil（Cg1）, surfaces of fruit body（Cg2） and endo-sclerotia（Cg3） belonged to four phyla [Ascomycota, Basidiomycota, Zygomycota and Rozellomycota] and some undetermined fungi, 17 classes, 43 orders, 77 families, 129 genera and 177 species. At genus level, the dominant genus in Cg1 was Mortierella, with relative abundance of 84.86%, followed by an uncertain genus（4.58%） and a genus of Chaetomiaceae（1.22%）; the dominant genus in Cg2 was Metacordyceps neogunnii（83.66%）, followed by Clonostachys rosea（9.48%）, Sarocladium（2.07%） and Mortierella spp.（1.12%）. The fungi in endo-sclerotia from Yifeng of Jiangxi（JX）, and Shiqian（SX）, Fenggang（FG）, and Shibing of Guizhou（Cg3） possess the same dominant species, i.e. M. neogunnii, and the relative abundances were 99.74%, 99.50%, 99.73% and 98.82%, respectively. The relative abundances of the rest genera were less than 1%. The dominant genus colonized in a dried specimen of M. neogunnii from Meitan was an uncertain genus in Clavicipitaceae, and its relative abundance was up to 99.97%. Both the stroma and endo-sclerotia of M. neogunnii dried specimen from Shibing were colonized by the same dominant species, M. neogunnii, with the relative abundances of 99.96% and 99.38%, respectively. In conclusion, the endo-sclerotia of the specimens of M. neogunnii from four areas were multiply colonized by some other fungi. However, M. neogunnii did not exist in the habitat soil, and the dominant genera in the habitat soil were Mortierella and Trichoderma. The specimen similar to M. neogunnii from Meitan was actually not conspecific, but belonged the other uncertain genus of Clavicipitaceae, which was in need of further study on its taxo

关 键 词：新古尼异虫草 内菌核 定殖真菌 宏条码技术 

分 类 号：Q949.32[生物学—植物学]