三种白腐真菌脱色噻嗪染料亚甲基蓝  

Decolorization of thiazine dye methylene blue by three white-rot fungi

作　　者：张桐 张波[1] 李玉[1] ZHANG Tong;ZHANG Bo;LI Yu(Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University,Changchun,Jilin 130118,China)

机构地区：[1]食药用菌教育部工程研究中心吉林农业大学,吉林长春130118

出　　处：《菌物学报》2018年第9期1243-1250,共8页Mycosystema

基　　金：现代农业产业技术体系建设专项资金(CARS20)~~

摘　　要：本研究通过含亚甲基蓝染料的固体培养基,从19株白腐真菌菌株中分离获得3个脱色能力较强的菌株,其在平板上的脱色圈大小分别为7.5cm、6.8cm和5.5cm。鉴定其为:云芝栓孔菌Trametes versicolor(ZT-197),绒毛栓孔菌Trametes pubescens(ZT-230)和亚黑管孔菌Bjerkandera fumosa(ZT-307)。其中,ZT-230对染料亚甲基蓝的脱色能力最强,可以将染料浓度为50mg/L的100m L液体培养基在6d之内100%脱色,而ZT-197和ZT-307在接种第10天时的脱色率为98%和80%。同时测定了3株白腐真菌在降解染料过程中的漆酶、锰过氧化物酶和木素过氧化物酶3种酶活力的规律:ZT-197和ZT-230均可分泌Lac和Mn P两种酶,ZT-307只分泌Li P。本研究说明绒毛栓孔菌ZT-197在印染废水治理方面具有较好的应用前景。Three isolates with ability to decolorize methylene blue dye were screened from among 19 white-rot fungal isolates cultured in solid media demonstrating decolorant zone of 7.5, 6.8 and 5.5 cm in diameter. They were identified as Trametes versicolor（ZT-197）, Trametes pubescens（ZT-230） and Bjerkandera fumosa（ZT-307）. ZT-230 showed the highest decolorizing ability, with decolorization rate of 100% for 100 m L liquid media containing 50 mg/L of methylene blue within six days, followed by ZT-197 with 98% within ten days and ZT-307 with 80% within ten days. Activities of extracellular enzymes（laccase, manganese peroxidase and lignin peroxidase） were also monitored. The results showed that ZT-197 and ZT-230 could secrete laccase and manganese peroxidase while ZT-307 lignin peroxidase only. Trametes pubescens ZT-230 has potential for dyeing effluent treatment application.

关 键 词：白腐真菌 亚甲基蓝 染料脱色 漆酶 锰过氧化物酶 木素过氧化物酶 

分 类 号：X703[环境科学与工程—环境工程] X172