转基因蚕丝丝素膜的制备与性能表征  

Preparation and Property Characterization of Transgenic Silk Fibroin Film

作　　者：赵兵 刘家豪 汪涛[1] 李智[1] 代方银[2] 赵天福[1] Zhao Bing;Liu Jiahao;Wang Tao;Li Zhi;Dai Fangyin;Zhao Tianfu(College of Textile ＆ Garment,Southwest University,Chongqing 400716,China;College of Biotechnology,South-west University,Chongqing 400716,China)

机构地区：[1]西南大学纺织服装学院,重庆400716 [2]西南大学生物技术学院,重庆400716

出　　处：《蚕业科学》2018年第4期560-566,共7页ACTA SERICOLOGICA SINICA

基　　金：重庆市蚕桑产业发展补助资金项目(No.CQ2017CSE011)

摘　　要：以转入蜘蛛丝蛋白基因的转基因家蚕的蚕丝为材料,用普通的流延成膜法即可将转基因蚕丝丝素溶液制备成丝素膜。以分别加入或未加入1%甘油的转基因蚕丝丝素膜和普通蚕丝丝素膜作为测试样品,通过傅里叶变换红外光谱（ATR-FTIR）、X射线衍射光谱（XRD）及机械性能测试和扫描电子显微镜（SEM）观察等手段表征转基因蚕丝丝素膜的结构与性能变化。与含1%甘油的普通蚕丝丝素膜比较,含1%甘油的转基因蚕丝丝素膜仍旧含有较多的无规卷曲和α螺旋结构,膜的表面较为粗糙,水蒸气透过量增加了15%-20%,水中溶失率略有增加（为22%）,应变增加了1倍左右（为38.5%）,耐久性也更优（为164次±11次）。此外,转基因蚕丝丝素膜和普通蚕丝丝素膜与1%甘油共混后,膜的结构和性能均得到明显改善。研究结果表明,含蜘蛛丝蛋白的转基因蚕丝丝素膜的结构较松散,表面孔隙变大,力学性能改善,更具有作为生物医用材料应用的优势。Silk fibroin films were made of silk materials from transgenic silkworm incorporated with spider silk protein genes by casting method. Using transgenic and non-transgenic silk fibroin films with or without 1% glycerol as samples,the properties of silk fibroin films were characterized by ATR-Fourier Transform Infrared Spectroscopy（ ATR-FTIR）,X-ray Diffraction（ XRD）,mechanical property test and Scanning Electron Microscopy（ SEM）. As compared with the non-transgenic silk fibroin films with 1% glycerol,transgenic silk fibroin films with 1% glycerol still contained high numbers of random coils and α-helixes. Surface roughness of transgenic silk fibroin film was observed. The water vapor transmission rate was increased by 15%-20%; the water dissolution rate was slightly increased（ 22%）; the strain was nearly doubled（ 38. 5%）; and the durability was also better（ 164±11 times）. In addition,the structure and properties of both transgenic and non-transgenic silk fibroin films could be significantly improved after adding 1% glycerin. Results indicate that the transgenic silk fibroin films containing spider silk proteins have looser structure,enlarged pores on surface,and improved mechanical properties,showing great advantages for being used as biomedical materials.

关 键 词：蜘蛛丝蛋白 转基因蚕丝 丝素膜 表面形貌 结构 机械性能 

分 类 号：R318.08[医药卫生—生物医学工程] S886.9[医药卫生—基础医学]