用不同蛹虫草菌株和家蚕品种幼虫覆土栽培蚕蛹虫草的试验  

An Experiment on Cultivating Silkworm-fungus Symbiont Using Different Cordyceps militaris Strains and Silkworm Larvae of Different Varieties Overlaid with Soil

作　　者：张俊 颜新培 李一平 徐安英[3] 钱何英 黄仁志 蒋勇兵 蒋诗梦 邹湘月 邵元元 Zhang dun;Yan Xinpei;Li Yiping;Xu Anying;Qian Heying;Huang Renzhi;Jiang Yongbing;Jiang Shimeng;Zou Xiangyue;Shao Yuanyuan(The Sericultural Research Institute of Hunan Province,Changsha 410127,China;Key Laboratory of Silkworm and Mulberry Genetic Improvement,Ministry of Agriculture,Zhenjiang Jiangsu 212018,China;The Sericultural Research In-stitute,Chinese Academy of Agricultural Sciences,Zhenjiang Jiangsu 212018,China)

机构地区：[1]湖南省蚕桑科学研究所,长沙410127 [2]农业部蚕桑遗传改良重点实验室,江苏镇江212018 [3]中国农业科学院蚕业研究所,江苏镇江212018

出　　处：《蚕业科学》2018年第4期608-614,共7页ACTA SERICOLOGICA SINICA

基　　金：湖南省农业科技创新资金创新团队建设项目(No.2017XC01);2017年度农业部蚕桑遗传改良重点实验室开放课题(No.KL201709);现代农业产业技术体系建设专项(No.CARS-18)

摘　　要：采用覆土培育的方法人工栽培蚕蛹虫草,在相同的培育环境条件下以6株蛹虫草菌（Cordyceps militaris）菌株和4个家蚕品种的5龄第6天幼虫供试,筛选合适的菌株和蚕品种。同一个家蚕品种的幼虫接种不同蛹虫草菌株的感染率差异显著;接种相同蛹虫草菌株120 h时,家蚕二元杂交组合与四元杂交组合幼虫间的感染率差异显著,至168 h,仅接种菌株H24的2个家蚕二元杂交组合间幼虫的感染率差异显著,各家蚕品种幼虫接种其余菌株的感染率已无显著差异,家蚕品种7532×湘晖的幼虫接种菌株LP4的感染率最高达到93.33%,并且该菌株对4个家蚕品种幼虫的感染能力显著高于其他菌株。不同菌株接种各家蚕品种幼虫的发菌率无显著差异。各家蚕品种幼虫接种菌株H24和H7未获得子实体,接种菌株H10和LP4的出草率最高,平均值分别为84.73%和88.70%。供试菌株和家蚕品种均显著影响蚕蛹虫草中3种活性成分的含量,并且菌株的影响更显著：以菌株L2接种7532×湘晖的幼虫培育蚕蛹虫草中的虫草素含量最高;以菌株H0接种932·芙蓉×7532·湘晖的幼虫培育蚕蛹虫草中的腺苷含量最高;以菌株H0接种洞·庭×碧·波的幼虫培育蚕蛹虫草中的虫草多糖含量最高。综合各项试验成绩初步认为：蛹虫草菌L2菌株和LP4菌株具有较高的生产应用价值;家蚕品种直接影响菌株的感染速度,对培育蚕蛹虫草中活性成分的含量也有一定影响。In this study,silkworm-fungus symbiont was artificially cultured by soil overlying method under the same cultivation condition to screen for suitable Cordyceps militaris strains and silkworm varieties. Six Cordyceps militaris strains were inoculated to the 6 th day silkworm（ Bombyx mori） larvae of the 5 th instar from 4 different varieties respectively. There were significant differences on infection rates in the same silkworm variety inoculated with different Cordyceps militaris strains. When inoculating the same Cordyceps militaris strain to larvae from different varietiesfor 120 h,there were significant differences on infection rates between silkworm varieties of binary cross combination and quaternary cross combination; after 168 h,significant difference on infection rate was only found between two varieties of binary hybrid combination inoculated with Cordyceps militaris strain H24,and no significant differences were found between other Cordyceps militaris strains and silkworm varieties. The infection rate of Cordyceps militaris strain LP4 to silkworm hybrid combination 7532·Xianghui reached 93. 33%, and its infectivity to the four silkworm varieties was significantly higher than other Cordyceps militaris strains. Germination rates of different Cordyceps militaris strains inoculated into larvae of various silkworm varieties have no significant difference. All silkworm varieties inoculated with H24 and H7 strains didn＇t germinate silkworm-fungus symbiont,and the average silkworm-fungus symbiont formation rate of silkworm larvae infected with H10 and LP4 strains were the highest,reaching 84. 70% and 88. 70% respectively. Results showed that both Cordyceps militaris strains and silkworm varieties had significant effects on 3 active component contents in silkworm-fungus symbiont,and the influence of strain was more significant. Content of cordycepin in silkworm-fungus symbiont inoculated with L2 strain to larvae of 7532×Xianghui was the highest; content of adenosine in silkworm-fungus symbiont inoculated

关 键 词：蛹虫草菌 家蚕品种 蚕蛹虫草 感染率 出草率 活性成分 

分 类 号：S886.9[农业科学—特种经济动物饲养]