东北铁线莲不同提取部位的抗炎镇痛作用研究  

Study on Anti-inflammatory and Analgesic Effect of Different Fractions Extracted from Clematis manshurica Rupr

作　　者：田淑霞[1,2] 李长艳 张婧娴[1] 肖霄[3] 郭澄 杜江[2] 韩永龙[1,3] Tian Shuxia;Li Changyan;Zhang Jingxian;Xiao Xiao;Guo Cheng;Du Jiang;Han Yonglong(Department of Pharmacy,Shanghai Jiaotong University Affiliated Sixth People＇s Hospital,Shanghai 200233,China;Guiyang University of Chinese Medicine,Guiyang 550025,China;Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海交通大学附属第六人民医院药剂科,上海200233 [2]贵阳中医学院,贵阳550025 [3]上海中医药大学,上海201203

出　　处：《世界中医药》2018年第8期2019-2022,共4页World Chinese Medicine

基　　金：上海市科学技术委员会民生科技支撑计划专项(14401972002);上海市第六人民医院东院人才课题(2014010)

摘　　要：目的:研究和比较东北铁线莲不同提取部位的抗炎镇痛活性。方法:东北铁线莲经提取、萃取、浓缩得到石油醚部位、氯仿部位、乙酸乙酯部位和正丁醇部位,实验分为阴性对照组、阳性对照组和实验组,分别利用小鼠醋酸扭体疼痛模型和脂多糖诱导RAW264.7细胞炎性反应模型评估4个提取部位的镇痛活性和抗炎活性。结果:在镇痛实验中,乙酸乙酯部位对小鼠扭体的抑制率最高;抗炎实验结果显示,乙酸乙酯部位抑制NO的效果最为显著。结论:东北铁线莲不同部位的抗炎镇痛效果不同,其中乙酸乙酯部位抗炎镇痛活性最强。Objective： To study the anti-inflammatory and analgesic activities of different fractions extracted from Clematis manshurica Rupr. Methods： The parts of petroleum ether,chloroform,ethyl acetate and n-butanol were obtained by abstracting,extracting and concentrating. The experiment was divided into three groups： negative control group,positive control group and experimental group. In the study,mouse acetic acid writhing and lipopolysaccharide（ LPS） induced RAW264. 7 cell model were used to evaluate analgesic and anti-inflammatory activity of the four fractions. Results： In the experiment of analgesic,the inhibition rate of writhing was the most significant in the ethyl acetate fraction. The results of anti-inflammatory experiment suggested that the ethyl acetate fraction showed significant inhibitory effect on nitric oxide（ NO）. Conclusion： The anti-inflammatory and analgesic effects of different parts of Clematis mandshurica were different,and ethyl acetate fraction showed the most significant anti-inflammatory and analgesic activity.

关 键 词：东北铁线莲 提取部位 抗炎 镇痛 

分 类 号：R285.5[医药卫生—中药学]