发酵虫草菌粉多糖免疫活性实验研究  

Experimental Study on Immune Activities of Polysaccharides from Fermented Cordycepssinensis Powders

作　　者：蒋洪亮 赵修南 麻浩 何丽芳 单俊杰 王海南[1,4] Jiang Hongliang;Zhao Xiunan;Ma Hao;He Lifang;Shan Junjie;Wang Hainan(School of Pharmaceutical Sciences,Guizhou University,Guiyang 550025,China;Institute of Military Medicine,Academy of Military Sciences,Beijing 100850,China;Hunan University of Chinese Medicine,Changsha 410208,China;China Food and Drug Administration,Beijing 100053,China)

机构地区：[1]贵州大学药学院,贵阳550025 [2]军事科学院军事医学研究院,北京100850 [3]湖南中医药大学,长沙410208 [4]国家食品药品监督管理总局,北京100053

出　　处：《世界中医药》2018年第8期2032-2035,共4页World Chinese Medicine

摘　　要：目的:探究发酵虫草菌粉多糖的免疫活性。方法:发酵虫草菌粉通过水提醇沉、复溶、透析、浓缩、冷冻干燥得多糖,命名为CSMP-60,其提取条件:60℃水提、提取时间4 h、料液比1∶15、提取次数2次。以小鼠免疫细胞为材料,检测CSMP-60的免疫活性。结果:CSMP-60能促进脾细胞的增殖(P<0.05),明显促进小鼠腹腔巨噬细胞分泌TNF-α(P<0.05),有促进脾淋巴细胞分泌IFN-γ的趋势。结论:CSMP-60可能是发酵虫草菌粉产生免疫活性的效应物质之一。Objective： To investigate the immunological activity of polysaccharides from fermented Cordycepssinensis powders.Methods： Polysaccharides from fermented Cordycepssinensis powders were obtained through water extraction and alcohol precipitation,reconstitution,dialysis,concentration and freeze-drying,named after CSMP-60. Its extraction conditions： 60 ℃ water extraction,extraction time 4 h,solid-liquid ratio 1∶ 15,the number of extractions 2 times. The immune activity of CSMP-60 was measured using mouse immune cells. Results： CSMP-60 could promote the proliferation of spleen cells（ P〈 0. 05）,and significantly promote the secretion of TNF-α from mouse peritoneal macrophages（ P〈 0. 05）. It has a tendency to promote the secretion of IFN-γ by spleen cells. Conclusion： CSMP-60 may be one of the immunocompetence substances of fermented Cordycepssinensis powders.

关 键 词：发酵虫草菌粉 多糖 细胞增殖 脾细胞 巨噬细胞 免疫活性 肿瘤坏死因子 Γ-干扰素 

分 类 号：R285.5[医药卫生—中药学]