多囊卵巢综合征患者颗粒细胞自噬的发生及影响因素  

Autophagy of ovarian granulosa cells in polycystic ovary syndrome

作　　者：李小雪[1] 王桂泉 朱琴玲[2] 何亚琼[2] 王缘[2] 齐家 吾.哈斯西木克 孙赟[2] Li Xiaoxue;Wang Guiquan;Zhu Qinling;He Yaqiong;Wang Yuan;Qi Jia;Wu Hasiximuke;Sun Yun(Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics,Centre for Reproductive Medicine,Renji Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai 200135,China)

机构地区：[1]上海交通大学医学院附属仁济医院生殖医学中心上海市辅助生殖与优生重点实验室,200135 [2]上海交通大学医学院附属仁济医院生殖医学中心,上海市辅助生殖与优生重点实验室,200135

出　　处：《中华生殖与避孕杂志》2018年第7期531-535,共5页Chinese Journal of Reproduction and Contraception

基　　金：国家自然科学基金面上项目(81771648,81571499);科技部重点专项子课题(2017YFC1001403);国家973子课题(2014CB943300);上海市卫生系统优秀人才培养计划(2017BR015)：上海市教育委员会高峰高原学科建设计划(20161413)

摘　　要：目的探讨多囊卵巢综合征(polycystic ovary syndrome,PCOS)患者颗粒细胞自噬的发生及影响因素。方法取卵日收集行体外受精/卵胞质内单精子显微注射(IVF/ICSI)的PCOS患者和非PCOS患者的卵泡液和颗粒细胞,检测患者卵泡液性激素以及颗粒细胞自噬相关基因的表达,进一步将患者的颗粒细胞自噬相关基因与卵泡液性激素和临床结局等指标进行相关性分析。结果 PCOS患者颗粒细胞自噬相关基因BECN1(2.13±0.94)和ATG7(1.38±0.45)mRNA水平显著高于非PCOS组患者颗粒细胞(1.46±0.52,P=0.002;1.16±0.29,P=0.026)mRNA水平;PCOS患者卵泡液黄体生成素(LH)[(5.32±4.14)IU/L]、雌二醇(E2)[(990.36±390.53)mg/L]和睾酮(T)[(53.18±28.36)nmol/L]水平显著高于非PCOS组患者卵泡液[(2.38±1.34)IU/L,P=0.001;(715.61±325.18)mg/L,P=0.007;(24.44±15.12)nmol/L,P=0.000],PCOS患者卵泡液P4[(12.81±6.78)mg/L]水平显著低于非PCOS组患者[(17.77±9.60)mg/L,P=0.036]。相关性分析显示,颗粒细胞中BECN1 mRNA水平与血清基础T水平(r=0.490,P=0.000)、卵泡液T水平(r=0.329,P=0.031)、血清抗苗勒管激素(AMH)(r=0.337,P=0.018)均呈显著正相关。结论 PCOS患者颗粒细胞自噬可能存在异常激活,可能为PCOS发生的重要机制。Objective To investigate autophagy in granulosa cells and its related factors in patients with polycystic ovary syndrome （PCOS）. Methods Follicular fluid （FF） and ovarian granulosa cells from PCOS and non-PCOS patients who underwent in vitro fertilization/intracytoplasmic sperm injection （IVF/ICSI） were collected, and sex hormones in FF and the expression of autophagy-related genes in granulosa cells were detected. Then, correlations between the expression of autophagy-related genes in granulosa cells with sex hormones and clinical outcomes were analyzed. Results The mRNA levels of autophagy-related genes BECN1 （2.13±0.94） and A TG7 （1.38±0.45） in granulosa cells of PCOS patients were significantly higher than that in non-PCOS patients （1.46±0.52, P=0.002; 1.16±0.29, P=0.026）. Concentrations of luteinizing hormone （LH） [（5.32±4.14） IU/L], estradiol （E2） [（990.36±390.53） mg/L], testosterone （T） [（53.18±28.36） nmol/L] in FF of PCOS patients were also higher than those of non-PCOS patients [（2.38±1.34） IU/L, P=0.001; （715.61±325.18） rag/L, P=-0.007; （24.44±15.12） nmol/L, P=0.000]. Concentration of progesterone （P4） [（12.81±6.78） mg/L] in FF of PCOS patients was lower than that of non-PCOS patients [（17.77＋9.60） mg/L, P=0.036]. BECN1 mRNA abundance in granulosa cells and testosterone levels in serum （r=0.490, P=0.000） and FF （r=0.329, P=0.031）, serum anti- Mullerian hormone （AMH） （r=0,337, P=0.018） showed significantly positive correlations. Conclusion The activation of autophagy in granulosa cells in PCOS patients may be an important mechanism for the development of PCOS.

关 键 词：自噬 抗苗勒管激素(AMH) 雄激素 多囊卵巢综合征(PCOS) 

分 类 号：R711.75[医药卫生—妇产科学]