重组人生长分化因子-9蛋白对小鼠促排卵作用的研究  

Invivo effects of recombinant human growth differentiation factor-9 protein on ovulation induction in mice

作　　者：王会妍[1] 罗海宁[1] 姚汝强[1] 杜湧瑞[1] 张云山[1] Wang Huiyan;Luo Haining;Yao Ruqiang;Du Yongrui;Zhang Yunshan(Reproductive Medical Center,Tianjin Central Hospital of Gynecology Obstetrics,Tianjin 300010,China)

机构地区：[1]天津市中心妇产科医院生殖医学中心,300010

出　　处：《中华生殖与避孕杂志》2018年第7期564-568,共5页Chinese Journal of Reproduction and Contraception

基　　金：天津市卫生局科技基金(2013KY40)~~

摘　　要：目的探讨重组蛋白生长分化因子-9(GDF-9)对小鼠卵泡发育的作用。方法选取30只性成熟雌性昆明小鼠随机分为3组,每组10只。对照组小鼠腹腔注射0.5 mL生理盐水;尿促性腺激素(hMG)组注射hMG 10 IU;联合组注射hMG 10 IU联合重组蛋白GDF-9 10μg;48 h后所有小鼠腹腔注射人绒毛膜促性腺激素(hCG)10 IU。注射hCG后16～20 h处死小鼠,比较各组小鼠卵巢指数(卵巢相对重量/相对体质量)及获卵数,酶联免疫吸附法(ELISA)法检测小鼠血清雌二醇(E2)含量,并对卵母细胞进行体外受精,观察各组受精情况。结果 hMG组、联合组获卵数(28.9±10.2、36.5±13.0)、卵巢指数[(32.26±6.33)mg/100 g、(38.80±8.90)mg/100 g]、血清E2含量[(45.64±14.19)ng/L、(52.02±16.08)ng/L]均高于对照组[11.4±5.4、(26.11±7.16)mg/100 g、(25.03±13.62)ng/L],差异有统计学意义(P=0.001、P=0.034、P=0.029;P=0.000、P=0.028、P=0.014);尤以联合组获卵数最多、卵巢指数最大、E2含量最高,与hMG组相比差异亦有统计学意义(P=0.025、P=0.031、P=0.043);对照组、hMG组、联合组组间小鼠卵母细胞体外受精率相近,差异无统计学意义(P>0.05)。结论重组蛋白GDF-9可以辅助促性腺激素(Gn)有效增加小鼠促排卵获卵数,且对卵母细胞的正常受精能力无影响。Objective To explore the effect of recombinant protein growth differentiation factor-9 （GDF-9） on follicular development in mice. Methods Thirty eugamic KM mice were divided into three groups, which received human menopausal gonadotropin （hMG） 10 IU, hMG 10 IU with recombinant protein GDF-9 10μg （combination group） and normal saline （0.5 mL） i.p. （control group）, respectively; 48 h later, human chorinnic gonadotropin （hCG） 10 IU was injected into each mouse, 16-20 h later 30 mice were killed, their ovary weight and the number of follicles were compared. Enzyme linked immunosorbent assay （ELISA） was used to examine the level of serum estrogen （E2）, their oocytes were fertilized in vitro, and fertilization information was observed. Results The number of follicles （28.9±10.2, 36.5±13.0）, ovary index [（32.26±6.33） mg/100 g, （38.80±8.90） mg/100 g] and the level of serum E2 [（45.6±14.19） ng/L, （52.02±16.08） ng/L] after ovulation induction in hMG group and combination group were higher than those of control group [11.4±5.4, （26.11±7.16） mg/100 g, （25.03±13.62） ng/L], with statistical significances （P=0.001, P=0.034, P=0.029; P=0.000, P=0.028, P=0.014）, especially, and they were significantly higher in combination group than in hMG group （P=0.025, P=0.031, P=0.043）. There were no significant differences in fertilization rate among different groups （P〉0.05）. Conclusion Recombinant protein GDF-9 combined with gonadotropin （Gn） could effectively increase oocyte retrieval rate in mice, while had no influence on mouse oocytes fertilizability. Utilizing recombinant protein GDF-9 combined with Gn provide a new clinical idea for improving ovarian response to ovulation stimulation.

关 键 词：促排卵 生长分化因子-9(GDF-9) 促性腺激素 卵巢反应 

分 类 号：R714.8[医药卫生—妇产科学]