miR-30b对心肌细胞凋亡影响的研究  

Effect of miR-30b on cardiomyocyte apoptosis

作　　者：胡莹[1] 齐凡星[1] 张明[1] 于丽娜[1] 张晨峰[1] Hu Ying;Qi Fanxing;Zhang Ming;Yu Lina;Zhang Chenfeng(Department of Cardiovascular Medicine,Baoding First Central Hospital,Baoding 071000,China)

机构地区：[1]保定市第一中心医院心血管内科,071000

出　　处：《国际免疫学杂志》2018年第4期408-411,共4页International Journal of Immunology

摘　　要：目的 探讨miR-30b对大鼠H9C2心肌细胞凋亡的影响.方法 将大鼠H9C2细胞分为空白对照组、阴性对照组和miR-30b mimics三个组,按照LipofectamineTM 2000转染方法转染24 h,RT-PCR检测三个组的miR-30b表达;将H9C2细胞分为阴性对照组、miR-30b mimics组和miR-30b mimics＋H2 O2组,24 h转染后并采用Annexin V-FITC/PI双染法检测细胞凋亡,以Western bloting法检测各组天冬氨酸蛋白水解酶3（Cleaved Caspase-3）、β-连环蛋白（β-catenin）和c-myc蛋白表达.结果 阴性对照组miR-30b的mRNA表达（1.007±0.062）与空白对照组（1.000±0.059）差异无统计学意义（F=0.200,P=0.849）,而miR-30b mimics组miR-30b的mRNA表达（4.212±0.088）高于阴性对照组（F=72.929,P=0.000）和空白对照组（F=74.261,P=0.000）.阴性对照组、miR-30b mimics组和miR-30b mimics＋H2 O2组的细胞凋亡率分别为（11.35±0.88）％、（1.22±0.13）％和（5.83±0.41）％,miR-30b mimics组、miR-30b mimics＋H 2 O2组细胞凋亡率均低于阴性对照组（F=778.088,P=0.000,F=193.976,P=0.000）,而miR-30b mimics组细胞凋亡率低于miR-30b mimics＋H 2 O2组（F=689.257,P=0.000）.miR-30b mimics组和miR-30b mimics＋H 2 O2组Cleaved caspase3（F=351.384,P=0.000,F=55.762,P=0.000）、β-catenin（F=506.207,P=0.000,F=248.244,P=0.000）和c-myc蛋白（F=456.110,P=0.000,F=115.466,P=0.000）表达均低于阴性对照组,而miR-30b mimics组Cleaved caspase3（F=167.105,P=0.000）、β-catenin（F=221.693,P=0.000）和c-myc（F=127.825,P=0.000）蛋白表达低于miR-30b mimics＋H 2 O2组.结论 过表达miR-30b可降低H9C2心肌细胞凋亡,其机制与下调Wnt信号通路有关. Objective To investigate the effect of miR-30b on the apoptosis of rat H9C2 cardiomyo-cytes. Methods Rat H9C2 cells were divided into 3 groups：the blank control group,the negative control group and the miR-30b mimics group. The expression of miR-30b in 3 groups was detected by RT-PCR after Lipo-fectamineTM2000 transfection. H9C2 cells were divided into 3 groups：the negative control group,the miR-30b mimics group and miR-30b mimics ＋ H2 O2 group. The aApoptosis was detected 24 h after transfection,by An-nexin V-FITC/ PI double staining. and the Cleaved caspase-3,β-catenin,and c-myc protein were detected by Western blotting. Results The mRNA expression of miR-30b in the negative control group （1. 007 ± 0. 062） was not statistically different from that in the blank control group （1. 000 ± 0. 059）（F = 0. 200,P = 0. 849）,but the miR-30b mRNA in the miR-30b mimics group （4. 212 ± 0. 088）was significantly higher than that in the negative control group（F = 72. 929,P = 0. 000）and the blank control group（F = 74. 261,P = 0. 000）. The ap-optotic the negative control group,the miR-30b mimics group and the miR-30b mimics ＋ H 2O2 group were （11. 35 ± 0. 88）%,（1. 22 ± 0. 13）%,and （5. 83 ± 0. 41）%,respectively. The apoptoticsis rate cells in the miR-30b mimics and miR-30b mimics ＋ H2 O2 groups was were significantly lower than that of the negative con-trol group （F = 778. 088,P = 0. 000,F = 193. 976,P = 0. 000）. The apoptotic cells rate in the of miR-30b mim-ics group was were significantly lower than that of miR-30b mimics ＋ H2O2 group（F = 689. 257,P = 0. 000）. The miR-30b mimics group and miR-30b mimics ＋ H2O2 groups showed significantly lower levels of cleaved caspase-3（F = 351. 384,P = 0. 000,F = 55. 762,P = 0. 000）,β-catenin expression（F = 506. 207,P = 0. 000, F = 248. 244,P = 0. 000）,and c-myc protein（F = 456. 110,P = 0. 000,F = 115. 466,P = 0. 000）were signifi-cantly lower than that in the negative control group. The miR-30b mimics group sh

关 键 词：miR-30b 心肌细胞 凋亡 

分 类 号：R54[医药卫生—心血管疾病]