农杆菌介导大豆遗传转化影响因素研究进展  

Research Advances of Agrobacterium-mediated Transformation Affecting Factors in Soybean

作　　者：杨柳 于翠梅[1] 刘铭 赵明哲[1] 谢甫绨[1] YANG Liu;YU Cui-mei;LIU Ming;ZHAO Ming-zhe;XIE Fu-ti(Agronomy college of Shenyang Agroeultural University, Shenyang 110866, China)

机构地区：[1]沈阳农业大学农学院,辽宁沈阳110866

出　　处：《大豆科学》2018年第5期803-808,共6页Soybean Science

基　　金：辽宁省教育厅项目(L2015490);辽宁省科技厅项目(2015020762)

摘　　要：大豆基因工程育种和功能基因组分析亟需建立高效大豆转化体系。本文综述了近年来影响农杆菌介导大豆遗传转化体系的农杆菌因素、植物因素及培养条件等方面的进展,主要包括农杆菌菌株、菌株生长状态、大豆基因型、外植体类型、防御酶、内源激素及侵染和共培养方法等,为提高大豆遗传转化效率及基因工程育种等相关研究提供参考。There is an urgent need to establish a high-efficiency transformation system for soybean genetic engineering breed-ing and functional genome analysis. This article summarized research advances of affecting factors including Agrobacterium fac-tors, plants factors and culture conditions that affect Agrobacterium tumefaciens mediated transformation rencent years, mainly a-bout Agrobacterium strains, bacterial growth state, soybean genotypes, explant types, defense enzymes, endogenous hormones and the condition of infection and co-cuhure. It will would provide reference for correlational studies of improving soybean transformation efficiency and genetic engineering breeding.

关 键 词：大豆 农杆菌 转化 影响因素 

分 类 号：S565.1[农业科学—作物学]