鸡GM-CSF和IL-2增强表达新城疫病毒F蛋白的重组杆状病毒疫苗的免疫效果  

Efficacy enhancement of a Baculovirus-vectored Newcastle Disease Virus F protein vaccine by chicken GM-CSF and IL-2

作　　者：于航[1] 高冬妮[1] 申燕[1] 刘颖[1] 平文祥[1] 葛菁萍[1] Hang Yu;Dongni Gao;Yan Shen;Ying Liu;Wenxiang Ping;and Jingping Ge(Key Laboratory of Microbiology,College of Life Sciences,Heilongjiang University,Harbin 150080,Heilongjiang,China)

机构地区：[1]黑龙江大学生命科学学院微生物省高校重点实验室,黑龙江哈尔滨150080

出　　处：《生物工程学报》2018年第9期1442-1452,共11页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.31270143);黑龙江省高等学校科技创新团队"农业微生物发酵技术"(No.2012td009)资助~~

摘　　要：为比较鸡粒细胞-巨噬细胞集落刺激因子(Granulocyte macrophage colony stimulating factor,GM-CSF)及鸡白细胞介素2(Interleukin 2,IL-2)对杆状病毒疫苗的免疫增强效果,通过基因工程手段构建重组杆状病毒疫苗(Recombinant Baculovirus,BV)rBV-LMI-F,并联合GM-CSF及IL-2进行鸡体免疫。对中和抗体水平及细胞因子含量比较GM-CSF和IL-2的免疫增强效果进行对比。结果显示,在第一次免疫28 d或42 d后,GM-CSF联合免疫组可诱导鸡体产生更高的抗体和细胞因子水平(P<0.01)。表明鸡GM-CSF能更有效地刺激机体产生较强的抗体和细胞因子反应,提高重组杆状病毒疫苗的免疫效果。To compare with the effects of the GM-CSF and IL-2 used as adjuvants in the baculovirus vaccine, we used genetic engineering to construct the recombinant baculovirus r BV-LMI-F and with GM-CSF and IL-2 to immunized chickens. Then, we compared the concentration of the neutralizing antibody and cytokines to determine the immunostimulatory effects of GM-CSF and IL-2. GM-CSF induced higher levels of antibodies and cytokines in chickens at 28 d and 42 d post-vaccination. In conclusion, GM-CSF could elicit higher serum antibody and cytokines responses and improved the effects of Baculovirus vaccine.

关 键 词：新城疫 GM-CSF IL-2 联合免疫 免疫效果 

分 类 号：S852.4[农业科学—基础兽医学]