串联重复序列在玉米基因组中的特征及分布  

The Characteristics and Distribution of Tandem Repeats in Zea mays Genome

作　　者：赵志新 高利敏 Zhao Zhixin;Gao Limin(College of Biopharmaceutical and Food Engineering,Shangluo University,Shangluo,72600)

机构地区：[1]商洛学院生物医药与食品工程学院,商洛726000

出　　处：《分子植物育种》2018年第18期5876-5883,共8页Molecular Plant Breeding

基　　金：商洛市科技局计划项目(SK-2014-01-19);商洛学院科研基金项目(14SKY028)共同资助

摘　　要：串联重复序列(tandem repeats, TRs)在玉米基因组中广泛存在,对维持染色体结构和调节基因表达起到重要作用。从Phytozome (http://www.phytozome.net/)下载玉米全基因组序列和基因注解(Gene annotation)数据,然后使用串联重复序列分析工具(Phobos)检测TRs的密度变化、模体类型及其在基因内与基因间的位置分布情况。研究表明,5'UTR和启动子中的TRs密度最高,内含子(Intron)和编码区(CDS)中TRs密度相对较低。同时TRs主要分布于CDS两端,这可能与内含子剪接有关;在基因间隔区,串联重复序列的分布明显偏向于靠近基因。本研究对串联重复序列在植物基因组中的特征及作用及有借鉴作用。Tandem repeats（TRs） exist widely in the Zea mays genome, and play key roles in the maintenance of chromosome and regulation of gene expression. Genome and gene annotation data of Z. mays was downloaded from Phytozome（http：//www.phytozome.net/）, and then tandem repeat search tool（Phobos version 3.3.12） was utilized for TR density, motif and distribution detection. The results showed that TRs densities in 5＇UTR and promoter regions were much higher than others, yet densities were lower in intron and CDS regions. Moreover,more TRs were frequently detected in the ends of CDS, considering that the preference may have relationship with intron splicing; in intergenic regions, more TRs were detected to close the genes. The study would help our understanding on TR characteristics and functions in plant genomes.

关 键 词：串联重复序列 玉米 密度 分布 

分 类 号：S513[农业科学—作物学]