半枫荷叶片转录组特征研究  被引量:7

Study on transcriptome characteristic of Semiliquidambar cathayensis Chang

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作  者:田晓明[1] 曾玲珍[1] 颜立红[1] 蒋利媛[1] 向光锋[1] TIAN Xiaoming;ZENG Lingzhen;YAN Lihong;JIANG Liyuan;XIANG Guangfeng(Hunan Forest Botanical Garden,Changsha 410116,China)

机构地区:[1]湖南省森林植物园,湖南长沙410116

出  处:《湖南林业科技》2018年第5期40-50,共11页Hunan Forestry Science & Technology

基  金:湖南省自然科学基金资助项目(2018JJ3299);湖南林业科技计划项目(XLK201718;XLK201816)

摘  要:半枫荷为特色木本药用植物,药用历史悠久,具有活血通络、祛风除湿、止血化瘀等功效。为探索半枫荷活性成分生物合成的分子基础,本文通过Illumina HiSeq 4000高通量测序技术对半枫荷叶片开展转录组测序分析,共获得了112 361条Contigs,进一步组装得到93 602条unigenes。对半枫荷叶片转录组unigenes进行Blast分析,得到47 798个CDS片段;与Swiss-Prot数据库相比较,得到29 778个蛋白同源序列;与COG数据库比对,有19 279条unigenes被COG功能注释;进行GO功能分类分析,有29 330条unigene注释到GO数据库中;进行KEGG功能注释,发现16 621个unigenes参与了131条代谢通路;通过生物信息学分析,发现多个萜类化合物生物合成途径关键基因。另外,转录组序列中共检测到21 819个SSR位点,其中二核苷酸所占比例最高,达到了47. 93%。研究结果不仅为进一步挖掘半枫荷次生代谢产物合成途径的关键基因提供了丰富的基因资源,同时为半枫荷遗传多样性研究奠定了分子基础。Semiliquidambar cathayensis Chang is a characteristics of woody medical plant,which has a long history of medicinal use,with activating blood stasis,dispelling wind and eliminating dampness,removing stasis and stopping blood,and other effects. In order to explore the molecular basis of active ingredients biosynthesis,S. cathayensis Chang was analyzed by transcriptome sequencing with the Illumina HiSeq 4 000 high-throughput sequencing technique. There were 112 361 contigs were obtained,and 93 602 unigenes were further assembled. All the unigene were analyzed by Blast,47 798 segments of CDS were obtained. All the unigenes were compared with the Swiss-Prot database,and 29,778 protein homologues were detected. All the unigenes were compared with the COG database,19 279 unigenes were annotated with COG function. According to GO classification analysis,29 330 unigenes were annotated in GO database. KEGG functional annotation analyses showed that 16 621 unigenes were involved in 131 metabolic pathways. By bioinformatics analysis,many key genes in terpenoid biosynthesis pathway were found. In addition,a total of 21 819 SSR location were detected in the transcriptome sequences,of which the highest proportion of dinucleotides was 47. 93%. The results not only provide abundant gene resources for further digging the key genes in the secondary metabolic pathway of S. cathayensis Chang,but also laid the molecular foundation for the study on the genetic diversity of it.

关 键 词:木本药用植物 半枫荷 转录组 次生代谢 萜类化合物 

分 类 号:S792.99[农业科学—林木遗传育种]

 

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