添加GSH对氟中毒小鼠肾脏SOD和CAT活性的影响  

Effect of GSH on the Superoxide Dismutase and Catalase Activity in Kidney of Fluorosis Mice

作　　者：张慧慧[1] 李瑞 王婧 王晓菲 孙子龙[1] ZHANG Huihui;LI Rui;WANG Jing;WANG Xiaofei;SUN Zilong(College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学动物科技学院,山西太谷030801

出　　处：《山西农业科学》2018年第10期1631-1633,1657,共4页Journal of Shanxi Agricultural Sciences

基　　金：山西省高等学校大学生创新创业训练计划项目(2017113)

摘　　要：试验研究氟中毒对小鼠肾脏超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性的影响,并证明添加谷胱甘肽(GSH)后对其的缓解作用,建立氟化钠(Na F)+GSH模型,进行总蛋白、SOD和CAT的测定。结果表明,与对照组相比,100 mg/L NaF组降低了肾脏SOD和CAT活性,且SOD活性降低差异显著;与100 mg/L NaF组相比,添加GSH后,400 mg/kg GSH组和800 mg/kg GSH组极显著提高了SOD活性;而对于CAT,加入GSH后活性虽有所升高,但未发现明显变化。说明通过添加GSH可以缓解氟中毒引起的肾脏损伤。This experiment is mainly to study the effects of fluorosis on the activities of total superoxide dismutase （SOD）andcatalase （CAT）in the kidney of mice and to demonstrate the alleviating effects of glutathione. Sodium fluoride （NaF）＋ reducedglutathione（GSH）model was established to measure total protein, SOD activity and CAT activity. The results showed that compared withthe control group, SOD activity of 100 mg/L NaF group was significantly decreased. Compared with the 100 mg/L NaF group, SOD activityof 100 mg/L NaF ＋ 400 mg/kg GSH and 100 mg/L NaF＋ 800 mg/kg GSH were both significantly increased. However, the activity of CAThad no obvious in treatment groups. It suggests that GSH can alleviate the renal injury caused by fluoride.

关 键 词：氟中毒 肾脏 超氧化物歧化酶 过氧化氢酶 谷胱甘肽 

分 类 号：R995[医药卫生—毒理学]