NGF及其受体在雄鸡睾丸组织中的定位及表达  

Localization and Expression of Nerve Growth Factor and Its Receptors in the Testis of Rooster

作　　者：李丽芳[1] 贾球锋[1] 乔琳[2] 张晋[1] 李嘉威[2] 张瑞强[2] LI Lifang;JIA Qiufeng;QIAO Lin;ZHANG Jin;LI Jiawei;ZHANG Ruiqiang(College of Information,Shanxi Agricultural University,Taigu 030800,China;College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学信息学院,山西太谷030800 [2]山西农业大学动物科技学院,山西太谷030801

出　　处：《山西农业科学》2018年第10期1718-1721,共4页Journal of Shanxi Agricultural Sciences

摘　　要：分别取14,20,24,35周龄同批次放养土鸡的睾丸组织,一部分经液氮处理后提取总蛋白,通过Western blot技术检测其表达量;另取一部分通过免疫组织化学技术对目的蛋白进行定位分析,研究神经生长因子(Nerve Growth Factor,NGF)及其受体NGFR(P75)在雄鸡睾丸组织中的定位及表达情况。结果表明,NGF及P75蛋白在各发育阶段的雄鸡睾丸组织内均有表达;随着日龄增加,表达部位有所变化,且NGF及P75的蛋白表达量随各发育阶段呈增加趋势。研究结果对于阐明NGF及其受体在禽类生殖发育中调节作用的分子机理有重要意义。The testes of 14, 20, 24, 35-week-old roosters were taken out from the same batch. One part of the testes were used toextract the total protein by Western blot to detect the expression of the proteins after freezed in liquid nitrogen. The other part of the testeswere analyzed by immunohistochemisty to study localization of target proteins, then to determine and discuss the localization and theexpression of NGF and its receptor NGFR（P75）in the testes of roosters. The results showed that NGF and P75 proteins expressed in thetesticular tissues during different developmental stages. With the increase of age, the expression parts had changed, and the expressionquantities of NGF and P75 kept raise. The results of this study are important for elucidating the molecular mechanism of NGF and itsreceptors in avian reproductive development.

关 键 词：神经生长因子 神经生长因子受体 雄鸡睾丸组织 免疫组织化学 蛋白免疫印迹法 

分 类 号：S831.1[农业科学—畜牧学]