小麦抗逆相关基因TaGB1的克隆及其表达特性分析  

Cloning and Expression Analysis of Resistance-Related Gene TaGB1 in Wheat

作　　者：王小婷 王文静[3] 李波 杨雯晶[2] 胡卫国[1] WANG Xiaoting1,2, WANG Wenjing3, LI Bo2 , YANG Wenjing2 , HU Weiguo1(1. Institute of Wheat, Henan Academy of Agricultural Sciences,/Henan Key Laboratory of Wheat Biology/Key Laboratory of Wheat Biology and Genetic Breeding in Central Huang Huai Region. Ministry of Agriculture/National Laboratory of Wheat Engineering. Zhengzhou, Henan 450002,China;2. College of Agronomy, Northwest A＆F University, State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, Shaanxi 712100 .China; 3. Shaanxi Agricultural Machinery Appraisal and Extension Station, Xian, Shaanxi 710003 ,China)

机构地区：[1]河南省农业科学院小麦研究所/河南省小麦生物学重点实验室/农业部黄淮中部小麦生物学与遗传育种重点实验室/国家小麦工程实验室,河南郑州450002 [2]西北农林科技大学农学院/旱区作物逆境生物学国家重点实验室,陕西杨凌712100 [3]陕西省农业机械鉴定推广总站,陕西西安710003

出　　处：《麦类作物学报》2018年第9期1011-1019,共9页Journal of Triticeae Crops

基　　金：国家转基因生物新品种培育重大专项(2016ZX08002002-010);陕西省重点研发项目计划(2018NY-026)

摘　　要：为了解小麦的TaGB1基因特性、表达情况及其与双子叶植物同源基因的进化关系,以小麦品种济麦22为研究对象,采用同源克隆的方法获得小麦G蛋白β亚基编码区序列,TaGB1编码区全长1 143bp,编码380个氨基酸,预测分子量为41kD,基因组序列中包含6个外显子和5个内含子,分别位于小麦基因组的4A、4B、4D染色体上,不同拷贝的氨基酸同源性高达99.91%。TaGB1基因结构中包含7个WD40保守域,表达产物位于胞质和质膜上。经系统发育进化关系分析,单子叶植物与双子叶植物的G蛋白β亚基分化形成两大分支;TaGB1在进化关系上与单子叶植物较近,而与拟南芥等双子叶植物较远。TaGB1在ABA、盐、热和干旱胁迫条件下上调表达,植物的根、茎、叶等部位均有表达,叶片中表达量较高,说明该基因可能参与调控植物的抗逆反应。To understand the genetic characteristics and expression of TaGB1 in wheat and its evolu- tionary relationship with the homologous genes of dicotyledonous plants, we cloned TaGB1 encoding wheat G protein/3 subunit by using Jimai 22 as experimental material,and the coding region of TaGB1 is 1 143 bp in length,encoding 380 amino acids. The predicted molecular weight was 41 kD. The ge- home sequence contained six exons and five introns,which were located in the A,B,D chromosomes of the wheat genome, and the amino acid homology was up to 99. 91%. The gene structure of TAGB1 contains seven-WD40 domains,and subcellular localization of TaGB1 was on plasma membrane and cy- toplasm. Phylogenetic evolutionary relationship indicated that the G protein β subunit of monocotyle- donous and dicotyledonous plants is divided into two large different branches and TaGB1 is close to monocotyledonous plants and alienated with dicotyledonous plants, such as ArabidoPsis thaliana. TaGB1 was up-regulated under ABA, salt, high hot and drought stress,and expressed in roots,stems and leaves of plants. Higher expression level was detected in leaves,indicating that TaGB1 may be in- volved in the regulation of plant stress response.

关 键 词：小麦 抗逆 TAGB1基因 同源克隆 表达 

分 类 号：S512.1[农业科学—作物学] S330