两种促渗方式对虫草素的透皮影响的实验研究  

The Comparison Study of Permeation Enhancing Effects of Microneedle and Azone on the Transdermal Absorption of Cordycepin

作　　者：王雪艳[1,2] 黄艳萍 杨莹 许剑怡 王宏娟[2] 唐旭东[1,2] WANG Xue-yan;HUANG Yan-ping;YANG Rong;XU Jian-yi;WANG Hong-juan;TANG Xu-dong(Key Lab for New Drug Research of TCM in Shenzhen,Research Institute of Tsinghua University in Shenzhen,Shenzhen,Guangdong,China,518000;Research Institute of Tsinghua University in Shenzhen-HuaYan Union Skin Healthcare Research Center,Shenzhen,Guangdong,518000)

机构地区：[1]深圳清华大学研究院创新中药及天然药物研究重点实验室,深圳广东5518000 [2]深圳清华大学研究院-华研共建皮肤健康研究中心,深圳广东518000

出　　处：《中国医疗美容》2018年第9期84-87,共4页China Medical Cosmetology

基　　金：深圳市科技创新项目JSGG20160301100442775;JCYJ20160301100720906

摘　　要：目的以含角质层的离体大鼠腹皮为体外模型,通过Franz透皮系统,考察微针、促渗剂两种促渗方式对虫草素透过角质层的影响。方法建立了高效液相色谱法测定虫草素方法,并测定了接收液中虫草素的含量,通过计算虫草素单位面积累积渗透量Q、稳态渗透常数J、增渗倍数ER、滞后时间Tlag,并建立了渗透模型,比较出微针组、促渗剂组、虫草素组的差异。结果虫草素透皮行为符合零级动力学模型。与未处理的虫草素组相比,150μm微针组和0.2%氮酮组均能显著增加虫草素单位面积累积渗透量,并提高经皮渗透速率(P <0.01),渗透行为符合零级动力学模型(J分别为0.8929,0.8025),即虫草素以恒定的速率穿过。其中,150μm微针组和0.2%氮酮组存在组间差异,但差异不显著(P <0.05),对应时间点下的微针组的渗透效率略优于0.2%氮酮组,可通过进一步研究优化实验参数。Objective To investigate the effect of different preparation enhancers on percutaneous absorption of cordycepin. Methods Using Franz-type diffusion cell and excised big mouse skin vitro as transdermal barrier, the content of ccs was determined by HPLC, then values of cumulative permeation quantity （Q）, the penetration rate （J） , enhancing rate（ER）, lag time （Tlag） were calulated. Conclusion The cumulative permeation quantity of cordycepin was signifcantly increased by microneedle and azone （P〈0.01）. The ordering of effect of enhancer on permeation quantity was 150μm microneedle 〉 Azone, but the values of them have no signifcant differences between the two groups （P〈0.05）. The release mechanism of CCS in vitro may be described by apparent zero order kinetics.

关 键 词：虫草素 透皮吸收 微针 氮酮 

分 类 号：R284[医药卫生—中药学]