苗药血人参的HPLC指纹图谱研究  

A Study on HPLC Fingerprint of the Miao Medicine Indigoferae stachyoidis

作　　者：刘梦鸽 徐宏[1] 徐文芬[1] 孙庆文[1] 王波[1] 陈胤睿 LIU Mengge;XU Hong;XU Wenfen;SUN Qingwen;WANG Bo;CHEN Yinrui(Guiyang University of Traditonal Chinese Medicine,Guiyang 550002,Guizhou China)

机构地区：[1]贵阳中医学院,贵州贵阳550002

出　　处：《贵阳中医学院学报》2018年第5期47-53,共7页Journal of Guiyang University of Chinese Medicine

基　　金：贵州省省长基金项目;项目编号:黔省专合字[2011]61号;贵阳中医学院大学生创新计划项目;项目编号:贵中医大创合字(2015)10号;国家工程技术研究中心组建项目;项目编号:2014FU125Q09

摘　　要：目的:建立苗药血人参药材的HPLC指纹图谱分析方法,为提高该药材的质量控制水平提供理论依据。方法:采用高效液相色谱法,以Phenomenex Synergi 4μHydro-RP 80A(250×4.6mm)为固定相;流动相为甲醇-乙腈-水,梯度洗脱,流速1.0m L/min;检测波长285nm;柱温30℃。测定分析贵州产16批血人参药材的指纹图谱,采用相似度评价、聚类分析、主成分分析3种模式识别方法进行指纹图谱分析比较。结果:测定的血人参药材指纹图谱有7个共有峰,共有峰的相对保留时间RSD为0.063%~0.43%,较全面的反映该药材中化学成分的信息。结论:建立的血人参HPLC指纹图谱测定方法,具有良好的稳定性和重复性,为苗药血人参药材的质量控制提供了参考依据。Objective： To establish the HPLC fingerprint analysis method of Indigoferae stachyoidis to provide a theoretical basis for improving the level of its quality control. Methods： HPLC was used. The HPLC chromatographic column was Phnomenex Synergi 4μ Hydro-RP 80 A（ 250 × 4. 6 mm）. The mobile phase was methanol-acetonitrile-water with gradient elution,and the flow rate was 1. 0 ml/min,detection wavelength of285 nm and column temperature 30 ℃. The fingerprints of 16 batches of Indigoferae stachyoidis produced in Guizhou were determined and analyzed. The similarity evaluation,cluster analysis and principal component analysis were used to compare the fingerprint patterns. Results： There were 7 common peaks in the fingerprints of Indigoferae stachyoidis. The relative retention time RSD of the common peak was 0. 063% - 0. 43%,which fully reflected the information of chemical composition in this medicine. Conclusion： The HPLC fingerprint method established for the determination of Indigoferae stachyoidis is stable and repeatable,which provides a reference for the quality control of this medicine.

关 键 词：苗药 血人参 指纹图谱 高效液相色谱法 质量控制 

分 类 号：R284.1[医药卫生—中药学] R29[医药卫生—中医学]