土壤微生物宏基因组文库构建及聚酮合酶基因的筛选  

Construction of a Soil Microorganism Metagenomic Library and Screening of Polyketide Synthase Genes

作　　者：杨雨蒙 徐敬国 胡伊旻 史雅凝 辛志宏[1] YANG Yu-meng;XU Jing-guo;HU Yi-min;SHI Ya-ning;XIN Zhi-hong(College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学食品科技学院,江苏南京210095

出　　处：《土壤通报》2018年第4期807-812,共6页Chinese Journal of Soil Science

基　　金：2017年农产品质量安全风险评估项目(GJFP201701101);江苏省"青蓝工程"资助

摘　　要：土壤中包含丰富的聚酮合酶(PKS)生物合成基因簇,其编码合成的聚酮化合物具有抑菌、抗病毒、抗肿瘤等多种生物活性,在食品、医药、化工等领域具有广泛的应用。为发现新型PKS基因,本研究采用宏基因组技术,以采自大连地区的土壤为研究对象,构建土壤宏基因文库,分别根据Ⅰ型PKS和Ⅱ型PKS结构域保守序列设计探针,PCR靶向筛选到4个含有PKS基因的单克隆,经测序与构建系统发育树分析,发现这4个单克隆的PKS基因分别单独聚为一支,与已知菌株编码的聚酮化合物亲缘关系较远,提示这些单克隆具有合成结构新颖聚酮化合物的潜力。本研究结果拓展了天然产物的开发利用途径,为新型聚酮化合物的发掘以及新药的获得提供了新思路。Soil is an important source of polyketide synthase（PKS） biosynthetic gene clusters, and the polyketide compounds which encoded have a variety of biological activities such as antibacterial, anti-virus and anti-tumor,which have been widely used in the fields of food, medicine and chemical industry. To discover new PKS genes, based on the metagenomic technology, we constructed soil（from Dalian region） mategenomic library, and used the conserved sequences of type Ⅰ PKS and type Ⅱ PKS domains as probes to amplificate targeted genes, and then screened clones containing the target genes. four PKS specific clones recovered were distantly related to known strains＇ polyketide synthase genes and may be encoded to novel and unique polyketides.. The results from the current research expand the exploitation and utilization of natural products and provide new ideas for the discovery of new polyketides and the acquisition of new drugs.

关 键 词：土壤 宏基因文库 序列筛选 聚酮合酶 

分 类 号：Q93[生物学—微生物学]