暗紫贝母甲羟戊酸途径关键酶HMGR的生物信息学分析  

Bioinformatic Analysis of Mevalonate Pathway Key Enzyme HMGR in Fritillaria unibracteata

作　　者：许勇[1] 戴陈伟 蔡标[1] XU Yong, DAI Chenwei, CAI Biao(Institute of Microbiology, Anhui Academy of Medical Sciences, Hefei 230061, China)

机构地区：[1]安徽省医学科学研究院微生物研究所,合肥230061

出　　处：《河南科学》2018年第9期1388-1394,共7页Henan Science

基　　金：安徽省"十三五"医疗卫生重点专科"病原微生物实验室"(皖卫科教[2017]30号);安徽省卫生计生委中医药科研课题"皖贝母内生真菌分离与鉴定"(2014zy46)

摘　　要：以暗紫贝母的3-羟基-3-甲基戊二酰辅酶A还原酶氨基酸序列为研究对象,利用CD search、Cell-PLoc和SWISS-MODEL等生物信息学在线预测软件对其保守区域、理化性质、亚细胞定位、结构和活性区域等性质进行分析.结果表明,该酶由559个氨基酸残基组成,是主体部分位于内质网膜外的二次跨膜疏水蛋白质.其二级结构主要由α-螺旋和无规则卷曲构成.三级结构为同源四聚体结构,活性区域由两条多肽链的66个氨基酸残基构成,活性区域的面积和体积分别为1 284.340?~2和2 152.618?~3.生物信息学手段分析预测结果揭示了暗紫贝母中3-羟基-3-甲基戊二酰辅酶A还原酶的性质、结构和功能,为进一步研究贝母活性生物碱的生物合成调控机制奠定理论基础.By using the amino acid sequence of 3-hydroxy-3-methylglutaryl-CoA reductase in Fritillaria unibracteata as the objects of this study,the conserved domains,physicochemical property,subcellular localization,structure andactive region were analyzed by online bioinformatic prediction tools including CD search,Cell-PLoc,SWISS-MODELand so on. The 3-hydroxy-3-methylglutaryl-CoA reductase,consisting of 559 amino acid residues,was an hydrophobicprotein with two transmembrane structures,which was mainly outside endoplasmic reticulum membrane. Alpha helixand random coil were main secondary structure components. The tertiary structure model was a homo-tetramer.The active region was consisted of 66 amino acid residues of two polypeptide chain,and the area and volume were 1 284.340 ？-2 and 2 152.618 ？-3. The characteristic,structure and function of the 3-hydroxy-3-methylglutaryl-CoAreductase in Fritillaria unibracteata were uncovered,which offered theoretical basis for the further study on regulatorymechanism of active alkaline biosynthesis in Fritillaria unibracteata.

关 键 词：暗紫贝母 3-羟基-3-甲基戊二酰辅酶A还原酶 生物碱 生物信息学 

分 类 号：R932[医药卫生—生药学] R318.04[医药卫生—药学]