典型复杂岩性油藏的微生物多样性及应用研究  

The Research and Application of Microbial Communities in a Model Complex Lithologic Reservoir

作　　者：苏三宝 董浩[2] 喻高明[1] 佘跃惠 SU Sanbao1, DONG Hao2, YU Gaoming1, SHE Yuehui1(1. School of Petroleum Engineering, Yangtze University, Wuhan 430100, China; 2. School of Chemistry and Environmental Engineering, Yangtze University, Jingzhou 434023,China)

机构地区：[1]长江大学石油工程学院,武汉430100 [2]长江大学化学与环境工程学院,湖北荆州434023

出　　处：《河南科学》2018年第9期1438-1443,共6页Henan Science

基　　金：国家自然科学基金项目(51474034)

摘　　要：为了拓展复杂岩性油藏的开采新方法,增强微生物提高原油采收率(MEOR)的可预测性.使用16S rDNA基因克隆文库方法,对典型复杂岩性油藏狮子沟油藏的微生物多样性进行了分析.结果表明,采油井的优势菌为盐单胞菌、肠杆菌、海杆菌、假单胞菌和不动杆菌;注水井的优势菌为海杆菌、盐单胞菌、脱硫盐单胞菌和弯枝菌.主成分分析(PCA)对狮子沟油藏样品实现聚类分组.系统发育分析表明采油井中的微生物主要为生物表活剂生产菌,而注水井中的微生物主要为石油烃降解菌;因此最适MEOR方式为采油井单井吞吐和注水井微生物解堵增注.研究结果有助于在复杂岩性油藏开展MEOR应用.To enhance oil recovery from complex lithologic reservoirs and boost the predictability for microbial enhanced oil recovery（MEOR）,the microbial communities of samples from a model complex lithologic reservoir were revealed by 16S rDNA gene clone library method.The results showed that the dominant bacteria in oil production wells were Enterobacter,Halomonas,Marinobacter,Pseudomonas and Acinetobacter.The dominate ones in water injection wells were Marinobacter,Halomonas,Desulfosalsimonas and Flexistipes.These samples were clustered by PCA analysis.Phylogenetic analyses demonstrated that the majority microorganisms in oil production wells were bio-surfactant producers,and the main bacteria in water injection wells were hydrocarbon degraders.Therefore,single well huff and puff for oil production wells and bacteria removing residue oil to enhance water injection for water injection wells would be the most suitable MEOR application procedures.These findings could promote the MOER application in complex lithologic reservoirs.

关 键 词：复杂岩性油藏 主成分分析 系统发育分析 微生物提高原油采收率 

分 类 号：TE39[石油与天然气工程—油气田开发工程]