和厚朴酚对神经病理性疼痛小鼠背根神经节TTX-R钠电流的影响  

Effects of honokiol on TTX-R Na^+ currents in neuropathic pain mouse dorsal root ganglion neurons

作　　者：张诗嘉 黄云[2] 盛安琪 刘文涛[3] 张广钦[2] ZHANG Shi-jia;HUANG Yun;SHENG An-qi(Department of Clinical Pharmacy,China Pharmaceutical University,Nanjing 210009,China)

机构地区：[1]南京市第九中学,210018 [2]中国药科大学临床药学教研室,210009 [3]南京医科大学江苏省神经退行性疾病重点实验室,211166

出　　处：《中国现代药物应用》2018年第19期210-213,共4页Chinese Journal of Modern Drug Application

基　　金：国家自然科学基金资助项目(项目编号:81471142)

摘　　要：目的在小鼠坐骨神经慢性压迫性损伤(CCI)模型上,观察和厚朴酚(Honokiol,Hon)对急性分离的背根神经节(DRG)细胞河豚毒素不敏感型(TTX-R)钠电流的作用,探讨其镇痛机制。方法应用CCI方法诱导神经病理性疼痛,全细胞膜片钳技术记录钠电流。结果与正常组比转,CCI模型使TTX-R钠电流明显增加,激活曲线向左偏移4.8 m V,不改变稳态失活曲线。Hon 30 M明显抑制CCI模型TTX-R钠电流的幅度,使激活曲线恢复趋向正常范围,失活曲线较CCI组向左偏移8.9 m V。结论 Hon对CCI模型增加的TTX-R钠电流有抑制作用,并影响其动力学特征。Objective On chronic constriction injury（CCI） model of sciatic nerve in mice, to observe the effects of honokiol（Hon） on tetrodotoxin-resistant（TTX-R） Na＋ currents in acute isolated dorsal root ganglion（DRG） neurons and explore its mechanism of analgesia. Methods CCI method was used to induce neuropathic pain. Whole cell patch clamp technique was used to record sodium currents. Results Compared with nomal DRG neurons, the amplitudes of TTX-R Na＋ currents were increased obviously, the steady state activation curve was shifted to more negative potential by 4.8 m V, but not altering the inactivation curve of TTX-R Na＋ currents in CCI mice. The amplitude of TTX-R Na＋ currents in the CCI model was significantly inhibited by Hon 30 M, so that the activation curve returned to the normal range, and the deactivation curve was shifted to the left by 8.9 m V compared with the CCI group. Conclusion Hon inhibites TTX-R Na＋ currents in CCI model and affects its kinetic characteristics.

关 键 词：和厚朴酚 河豚毒素不敏感型钠电流 坐骨神经慢性压迫性损伤模型 

分 类 号：R285.5[医药卫生—中药学]