肝胃百合汤对慢性应激与幽门螺杆菌双重损伤因素模型小鼠胃黏膜组织TFF1、p-ERK蛋白表达的影响  

Effects of Ganwei Baihe Decoction on Protein Expressions of TFF1 and p-ERK in Gastric Mucosa of Model Mice with Chronic Stress and Helicobacter Pylori Double Injury Factors

作　　者：喻斌[1] 曾孟晖 徐寅[1] 阳力[1] 周赛男[1] 杜珊[2] 毛一之[2] 李珍[1] 李玲[1] YU Bin;ZENG Meng-hui;XU Yin;YANG Li;ZHOU Sai-nan;DU Shan;MAO Yi-zhi;LI Zhen;LI Ling(First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,China;Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学,湖南长沙410208

出　　处：《中国中医药信息杂志》2018年第10期44-48,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：湖南省中医药管理局重点课题(201512);湖南省教育厅科学研究重点项目(17A158)

摘　　要：目的观察肝胃百合汤对慢性应激与幽门螺杆菌(Hp)双重损伤因素模型小鼠胃黏膜组织三叶因子1(TFF1)、磷酸化细胞外信号调节激酶(p-ERK)蛋白表达的影响,探讨其治疗慢性应激性胃溃疡合并Hp感染的相关机制。方法将60只BALB/c雄性小鼠随机分为空白组、应激组、单纯Hp组、双重因素组、肝胃百合汤组、雷尼替丁组,每组10只,选取10种不可预知刺激因素制备慢性应激小鼠模型,造模第7日给予单纯Hp组、双重因素组、肝胃百合汤组、雷尼替丁组灌胃Hp菌液;肝胃百合汤组给予肝胃百合汤灌胃,雷尼替丁组给予雷尼替丁灌胃,其余各组给予蒸馏水灌胃。采用免疫组化检测小鼠胃黏膜组织TFF1、p-ERK蛋白表达。结果双重因素组溃疡指数与TFF1、p-ERK蛋白表达明显高于空白组(P<0.01);与双重因素组比较,肝胃百合汤组和雷尼替丁组溃疡指数显著降低(P<0.01),且肝胃百合汤组明显低于雷尼替丁组(P<0.01);肝胃百合汤组和雷尼替丁组TFF1、p-ERK蛋白表达明显高于双重因素组(P<0.05,P<0.01),且肝胃百合汤组明显高于雷尼替丁组(P<0.01)。结论慢性应激与Hp可协同致胃黏膜损伤,肝胃百合汤可促进TFF1、p-ERK的表达及胃黏膜的修复,达到促进溃疡愈合的目的。Objective To observe the effects of Ganwei Baihe Decoction on the protein expressions of TFF1 and p-ERK in gastric mucosa of model mice with chronic stress and Helicobacter pylori（Hp） double injury factors; To explore the relevant mechanism for treating chronic stress gastric ulcer complicated with Hp infection. Methods Totally sixty BALB/c male mice were randomly divided into blank group, stress group, Hp group, double factors group, Ganwei Baihe Decoction group, and ranitidine group, with 10 mice in each group. 10 unpredictable stimulus factors were selected to prepare chronic stress mouse models. On the 7 th day of modeling, Hp group, double factors group, Ganwei Baihe Decoction group, and ranitidine group were given Hp bacteria, Ganwei Baihe Decoction and ranitidine were given to corresponding groups for gavage, respectively, and the other groups were given distilled water for gavage. The protein expressions of TFF1 and p-ERK in gastric mucosa of mice were detected by immunohistochemistry. Results The ulcer index and protein expressions of TFF1 and p-ERK in the double factors group were higher than those of the blank group（P〈0.05, P〈0.01）; Compared with double factors group, the gastric mucosal injury index decreased in Ganwei Baihe Decoction group and ranitidine group（P〈0.01）, and Ganwei Baihe Decoction group was lower than ranitidine group（P〈0.01）; The protein expressions of TFF1 and p-ERK in Ganwei Baihe Decoction group and ranitidine group were significantly higher than those in double factors group（P〈0.01）, and Ganwei Baihe Decoction group was higher than ranitidine group（P〈0.01）. Conclusion Chronic stress and Hp can cause gastric mucosal injury in synergy; Ganwei Baihe Decoction can promote the repair of gastric mucosa and expressions of TFF1 and p-ERK, so as to enhance ulcer healing.

关 键 词：肝胃百合汤 慢性应激性胃溃疡 幽门螺杆菌 三叶因子1 磷酸化细胞外信号调节激酶 小鼠 

分 类 号：R285.5[医药卫生—中药学]