EV71感染手足口病患儿T细胞亚群表达  

Expression of T cell subsets in children with hand, foot and mouth disease caused by enterovirus 71 infection

作　　者：邹莉[1] 梅文静[1] Zou Li;Mei Wenjing(Department of Pediatrics,Huizhou City People＇s Hospital,Huizhou 516001,China)

机构地区：[1]惠州市中心人民医院儿科,惠州市516001

出　　处：《中华实验和临床感染病杂志（电子版）》2018年第4期397-401,共5页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

摘　　要：目的分析EV71感染手足口病患儿T细胞亚群的表达。方法选取2015年10月至2017年10月于惠州市中心人民医院接受治疗的手足口病患儿共302例,其中EV71感染患儿110例,依据EV71感染患儿中枢神经有无被累及分成轻症组(69例)与重症组(41例),选取同期于本院体检的健康儿童110例作为对照组;流式细胞仪对T淋巴细胞亚群CD3+、CD3+CD8-、CD3+CD8+、CD3+CD8-/CD3+CD8+、Th1、Th2、Th1/Th2、Tc1、Tc2、Tc1/Tc2、Th17、Tc17、调节性T淋巴细胞(Treg)及Th17/Treg所占比率进行检测,ELISA法检测各组对象血清内转化生长因子-β1(TGF-β1)、白细胞介素4(IL-4)、白细胞介素17A(IL-17A)及γ干扰素(IFN-γ)含量变化。结果轻症组患儿血清Th1、Th1/Th2、Tc1和Tc1/Tc2所占比率为(9.59±2.15)%、(8.67±2.19)%、(28.82±5.49)%和(56.39±10.48)%,重症组分别为(13.58±2.74)%、(9.45±2.29)%、(40.46±6.37)%和(54.87±9.61)%,均显著高于对照组患儿,差异均有统计学意义(F=12.159、11.470、13.925、10.542,P=0.016、0.013、0.008、0.035);轻症组患儿血清IFN-γ和IL-17A水平分别为(11.32±2.76)pg/ml和(6.38±2.10)pg/ml,重症组分别为(11.38±2.65)pg/ml和(12.59±3.58)pg/ml,均显著高于对照组患儿,差异均有统计学意义(F=12.590、11.664,P=0.024、0.019)。结论 EV71感染后手足口患儿机体内Th17/Treg与Th1/Th2比例失衡。Objective To investigate the expression of T cell subsets in children with hand, foot and mouth disease（HFMD） of EV71 infection. Methods From October 2015 to October 2017, 302 children with HFMD were treated in Huizhou City People＇s Hospital, including 110 cases with EV71 infection. According to whether or not the central nervous system was involved in children with EV71 infection, cases were divided into mild group（69 cases） and severe group（41 cases）, while 110 healthy children were selected as the control group. T lymphocyte subsets CD3^＋, CD3^＋CD8^-, CD3^＋CD8^＋, CD3^＋CD8^-/CD3^＋CD8^＋, Th1, Th2, Th1/Th2, Tc1, Tc2, Tc1/Tc2, Th17, Tc17 and regulatory T cells（Treg） and Th17/Treg ratio were detected by flow cytometry, respectively. The change of serum TGF-beta 1（TGF-β1）, interleukin-4（IL-4）, interleukin-17 A（IL-17 A） and interferon gamma（IFN-γ） were detected by ELISA. Results The levels of serum Th1, Th1/Th2, Tc1 and Tc1/Tc2 of cases in mild group were（9.59 ± 2.15）%,（8.67 ± 2.19）%,（28.82 ± 5.49）% and（56.39 ± 10.48）%; while those in severe group were（13.58 ± 2.74）%,（9.45 ± 2.29）%,（40.46 ± 6.37）% and（54.87 ± 9.61）%, respectively, which were all significantly higher than those of the control group, with statistically significant differences（F = 12.159, 11.470, 13.925, 10.542, P = 0.016, 0.013, 0.008, 0.035）. The levels of serum IFN-γ and IL-17 A levels of cases in mild group were（11.32 ± 2.76） pg/ml,（6.38 ± 2.10） pg/ml, while in severe group were（11.38 ± 2.65） pg/ml,（12.59 ± 3.58） pg/ml, which were all significantly higher than those of the control group, with significant differences（F = 12.590, 11.664; P = 0.024, 0.019）. Conclusion The proportion of Th17/Treg and Th1/Th2 in children with HFMD after EV71 infection is unbalanced.

关 键 词：手足口病 肠道病毒71型 T细胞亚群 

分 类 号：R725.1[医药卫生—儿科]