人参皂苷Rg3增强PD-1抑制剂对弥漫大B细胞淋巴瘤免疫治疗作用的体外研究  

Effect of Ginsenoside Rg3 Enhancing the Immunity of PD-1 Inhibitor in Treatment of DLBCL: A Study in Vitro

作　　者：郭奕维 郭秀臣[1] 张静波[1] 刘爱春[1] 全丽娜[1] GUO Yiwei;GUO Xiuchen;ZHANG Jingbo;LIU Aichun;QUAN Lina(Department of Hematology,the Affiliated Tumor Hospital of Harbin Medical University,Harbin 150081,China)

机构地区：[1]哈尔滨医科大学附属肿瘤医院血液淋巴内科,黑龙江哈尔滨150081

出　　处：《中医药学报》2018年第5期24-29,共6页Acta Chinese Medicine and Pharmacology

基　　金：黑龙江省中医药科研项目(ZHY16-108);哈尔滨医科大学附属肿瘤医院海燕科研基金项目(JJQN2016-04)

摘　　要：目的:探讨人参皂苷Rg3联合PD-1抑制剂对弥漫大B细胞淋巴瘤(DLBCL)的作用,通过体外实验观察人参皂苷Rg3在增强T细胞对抗淋巴瘤治疗中的作用及其机制。方法:收集DLBCL患者的外周血,提取单个核细胞,体外激活并扩增T细胞,建立T细胞与肿瘤细胞共培养体系,检测联合人参皂苷Rg3能否增强PD-1抑制剂对T细胞增殖、凋亡和细胞因子分泌的影响。结果:DLBCL细胞可抑制T细胞增殖,促进凋亡;联合人参皂苷Rg3能够增强PD-1抑制剂对恢复T细胞增殖,减少凋亡,并增加细胞因子IL-2和IFN-γ分泌的作用。结论:人参皂苷Rg3增强PD-1抑制剂对DLBCL的抗肿瘤作用,其机制为增强T细胞增殖,抑制凋亡,增加细胞因子分泌。Objective ： To investigate the effect of ginsenoside Rg3 combined with PD - 1 inhibitor in treatment of DLBCL,and to explore the role and mechanism of ginsenoside Rg3 in enhancing the function of T cells against lymphoma cells invitro. Methods： The peripheral blood of DLBCL patients were collected, mononuclear cells were extracted, and T cellswere activated and amplified in vitro. Then the cocuhure system of T cells and tumor cells was established to detectwhether ginsenoside Rg3 could enhance the effect of PD - 1 inhibitor on the proliferation, apoptosis and cytokine secre-tion of T cells. Results ： DLBCL cell could inhibit the proliferation of T cells and promote its apoptosis. When combinedwith ginsenoside Rg3, it could enhance the effect of PD - 1 inhibitor on regaining proliferation of T cells, reducing apop-tosis, and increasing the secretion of cytokine IL - 2 and IFN - γ. Conclusion： Ginsenoside Rg3 enhances the anti -tumor effect of PD - 1 inhibitor on DLBCL, which mechanism is enhancing the proliferation of T cells, inhibiting the ap-optosis, and increasing the secretion of cytokine.

关 键 词：人参皂苷RG3 PD-1抑制剂 弥漫大B细胞淋巴瘤 免疫治疗 

分 类 号：R273[医药卫生—中西医结合]