绞股蓝种子中新皂苷的提取及其消炎活性研究  

Research on extraction and anti-inflammatory activity of new saponins from Gynostemma pentaphyllum seeds

作　　者：周文超 Zhou Wenchao(School of chemical and chemical engineering,Ankang university,Ankang 725000,China)

机构地区：[1]安康学院化学化工学院,陕西安康725000

出　　处：《云南化工》2018年第8期34-37,共4页Yunnan Chemical Technology

摘　　要：为了充分利用绞股蓝种子资源,分析了绞股蓝种子活性成分,利用硅胶柱层析和半制备高效液相色谱技术得到一种三萜皂苷,并通过氢谱碳谱及二维核磁,高分辨质谱,红外光谱鉴定其结构,其结构为25-O-acetyl-cucurbitacin L 2-O-[α-rhamnopyranosyl(1→2)][α-L-arabinopyranosyl(1→3)]-β-glucopyranoside。通过对脂多糖诱导的小鼠巨噬细胞RAW 264症模型测试新化合物抑制炎症因子的能力,发现其具有一定的抗炎活性。For the sake of the seed resources of Gynostemma pentaphyllum, We analyzed the active ingredientsof gynostemma seeds, and the triterpenoid saponin was separated by silica gel column chromatography andpreparative high performance liquid chromatography technology.The structure was identified by 1H, 13C NMR,high resolution mass spectrum, infrared spectrum.The result indicated that this compound is a new compound thathas not been reported, it＇ s structure was dentified as 25-O-acetyl-eueurbitaein L 2-O-[α-rhamnopyranosyl （ 1-2 ） ][α-L-arabinopyranosyl （ 1-3 ） ]-β-glucopyranosideo.The new compound showed that the inhibitoryactivities on the expression of TNF-ct ml：{NAs in the lipopolysaeeharide-indueed RAW 264 mouse maerophage cellsmodel.

关 键 词：绞股蓝种子 三萜皂苷 结构鉴定 抗炎活性 

分 类 号：R284[医药卫生—中药学]