HIV-1感染者CD4^+CD127^-T细胞减少与PD-1高表达相关  

Loss of CD4^+CD127^-T cells during HIV infection associates with high PD-1 expression

作　　者：彭丽珊[1] 冷静[1] 刘显 杨洋[1] 王登嵘[1] 伍月榕 农清栋[1] 于丽 王盈[3] 肖健[1] PENG Lishan;LENG Jin;LIU Xian;YANG Yang;WANG Dengrong;WU Yuerong;NONG Qingdong;YU Li;WANG Ying;XIAO Jian(Department of Guangxi Key Labortary of High Incidence of Infectious Disease of Integrated Chinese Medicine and Western Medicine Transformation,Guangxi University of Chinese Medicine,Nanning 530200,China;Laboratory of Medical Molecular Biology of First Affiliated Hospital,Guangxi University of Chinese Medicine,Nanning 530023,China;Shanghai Center for Disease Control and Prevention,Shanghai 200336,China)

机构地区：[1]广西中医药大学广西高发传染病中西医结合转化医学重点实验室,南宁530020 [2]广西中医药大学第一附属医院医学分子生物学实验室,南宁530023 [3]上海市疾病预防控制中心,200336

出　　处：《免疫学杂志》2018年第10期886-893,共8页Immunological Journal

基　　金：国家自然科学基金(81260463);广西自然科学基金重点项目(2011GXNSFD018031);广西自然科学基金(2012G XNSFAA053120);广西中医药大学第一附属医院院级青年基金科学项目(2017NQ004);广西中医药大学引进博士科研启动基金(2017BS045)

摘　　要：目的通过检测HIV-1感染者CD4+CD127^-T细胞上PD-1、HLA-DR和CD38的表达水平,探究HIV-1感染者CD4+CD127^-T细胞的免疫状态。方法募集慢性HIV-1感染者70例,根据CD4^+T细胞计数绝对值,将慢性HIV-1感染者分为高CD4+计数组(CD4^+T细胞≥500 cells/μl)、中CD4+计数组(350 cells/μl≤CD4^+T细胞<500 cells/μl)和低CD4+计数组(CD4^+T细胞<350 cells/μl);同时设立健康对照组12例。利用流式细胞术检测HIV-1感染者和健康对照者外周血中CD4+CD127^-T细胞PD-1、CD38和HLA-DR的表达情况,并分析不同指标表达的相关性。结果在HIV-1慢性感染期间,外周血CD4+CD127^-T细胞百分比与CD4计数绝对值负相关(P<0.05);CD4+计数<350 cells/μl的HIV-1感染者CD4+CD127^-T细胞上HLA-DR与CD38表达水平显著升高,并且与CD4计数存在负相关关系(P<0.05)。HIV-1感染者PBMCs中CD4+CD127^-T细胞上高表达PD-1,并且PD-1与CD4计数存在负相关关系,与HLA-DR的表达存在正相关关系。结论在慢性HIV-1感染过程中,CD4^+T细胞数量的减少可能与CD4+CD127^-T细胞PD-1的表达相关。To investigate HIV-1 infection by detecting enrolled, and sub-categorized the relationship between the activation and exhaustion of CD4^＋ CDI27-T cells in the expression levels of PD-1, CD38, HLA-DR, total of 70 HIV-1 patients were by high, middle or low CD4^＋ T cell counts. PD-1 and immune activation markers CD38, HLA-DR were analyzed by flow cytometry. Data showed that the ratio of CD4^＋CD127^-T cells in the peripheral blood were negatively correlated with CD4 counts during chronic HIV-1 infection. When CD4^＋ counts were less than 350 cells/μl, CD38 and HLA- DR were highly expressed on CD4^＋CD127^- T cells, and negatively associated with peripheral CD4 T cell counts. PD-1 was highly expressed on CD4^＋CD127^-T cells, and negatively associated with peripheral CD4 T cell counts, while positively associated with HLA-DR. Taken together, PD-1 expressed on CD4^＋ CD127^-T cells may relate to the loss of CD4^＋T cells during chronic HIV infection.

关 键 词：HIV-1 CD4^+CD127-T细胞 PD-1 CD38 HLA-DR 

分 类 号：R512.91[医药卫生—内科学]