Phylogenetic analysis of click beetles(Coleoptera: Elateridae) based upon 28S rDNA: phylogeny and classification  

基于28S rDNA片段的叩甲科昆虫系统发育分析:系统发育与分类(鞘翅目:叩甲科)（英文）

作　　者：Ziye MENG Chaoliang LEI Xiaoqin CHEN Taoxiong SHI Qijiao CHEN Shihong JIANG 孟子烨;雷朝亮;陈晓琴;石桃雄;陈其皎;江世宏(贵州师范大学荞麦产业技术研究中心,贵州贵阳550001;华中农业大学昆虫资源研究所,湖北武汉430070;深圳职业技术学院化学与生物技术学院,广东深圳518055)

机构地区：[1]Research Center of Buckwheat Industry Technology,Guizhou Normal University,Guiyang,Guizhou 550001,China [2]Key Laboratory of Insect Resources Utilization ＆ Sustainable Pest Management of Hubei Province,Institute of Insect Resources,Huazhong Agricultural University,Wuhan,Hubei 430070,China [3]College of Applied Chemistry and Biotechnology,Shenzhen Polytechnic,Shenzhen,Guangdong 518055,China

出　　处：《Entomotaxonomia》2018年第3期231-252,共22页昆虫分类学报（英文）

基　　金：supported by the National Natural Science Foundation of China (31772511);Guizhou Science and Technology Cooperation Project(QianKeHe LH Zi [2016]7214);Earmarked Fund for Construction of the Key Laboratory for Conservation and Innovation of Buckwheat Germplasm in Guizhou(QianJiaoHe KY Zi [2017] 002);the Doctoral Research Project of Guizhou Normal University

摘　　要：Elateridae（click beetles） is the largest family within the Elateroidea and is abundant worldwide. However, their phylogenetic relationships remain uncertain; older studies based on the external morphology of adults and larvae led to divergent classification systems. Many tribes, genera, and even subfamilies remain controversial. Partial sequences of 28S ribosomal DNA（rDNA） were used to investigate the phylogeny of click beetles. Sequences of 28S rDNA from 80 different click beetle species collected from different locations across China were determined along with those of another 86 Elateridae species and 14 outgroup species. The aligned data were analyzed by neighbor joining, maximum parsimony, maximum likelihood, and Bayesian analyses. All methods produced a nearly consistent basal topology that was partially congruent with those of previous studies based on adult and larval morphology as well as molecular data. Overall, the monophyly of Elateridae was supported. The family was divided into five clades, Denticollinae, Negastriinae, Cardiophorinae, Pyrophorinae, Oestodinae, and Elaterinae. Senodonia is traditionally considered a member of Elateridae, but this study indicated that it was more closely related to Lycidae. The position of the outgroup family Lampyridae also differed from its placement in previous research. In addition to confirming the Elateridae as monophyletic, we concluded that Oxynopterinae, Pityobiinae, and Hypnoidinae should be merged into Denticollinae; Conoderinae and Agrypninae should be regarded as subgroups of Pyrophorinae; and Melanotinae should be classified as a tribe of Elaterinae. Additional species should be sampled in future studies to confirm this reclassification.叩甲科昆虫是叩甲总科中最大的一科,广泛分布于全世界。但是直到现在叩甲科的分类系统还存在着不确定的地方,因为早期的分类系统是基于成虫和幼虫的外部形态特征建立的,很多的族、属、甚至亚科的划分都有争议。本研究利用核糖体28S rD NA片段,对国内采集的叩甲科昆虫共80种进行系统发育分析。分析中还采用了Gen Bank已有的86种叩甲科昆虫序列作为内群,14种外群,利用邻接法、最大似然法、最大简约法和贝叶斯法构建系统发育树。不同方法构建的系统发育树结构相同,并部分支持了基于外部形态特征建立的分类系统。结果显示,叩甲科共分为5个分支,分别为齿胸叩甲亚科分支、小叩甲亚科分支、心盾叩甲亚科分支、萤叩甲亚科分支以及叩甲亚科分支。其中,方胸叩甲属属于叩甲科类群,在系统树中却更加接近于红萤科,并且外群中萤科的位置也与以前的观点不同。研究的结果支持叩甲科昆虫具有良好的单系性。并且根据结果提出以下假设:尖鞘叩甲亚科、异角叩甲亚科和胖叩甲亚科可以纳入齿胸叩甲亚科内部;单叶叩甲亚科和槽缝叩甲亚科并入萤叩甲亚科;梳爪叩甲亚科并入叩甲亚科。这个结论还需要引入更多的种类及分子标记来加以证实。

关 键 词：Elateroidea classification systems TAXONOMY 

分 类 号：Q969.48[生物学—昆虫学]