临床分离鲍氏不动杆菌生物膜形成能力与耐药性关系研究  

Relationship between ability of biofilm formation and drug resistance of clinical Acinetobacter baumannii isolates

作　　者：牛付轩 吴亮[2] 王廷廷 朱丽华[1] 段秀杰[1] 高雁[1] 张亚峰 阴晴[1] NIU Fu-xuan;WU Liang;WANG Ting-ting;ZHU Li-hua;DUAN Xiu-jie;GAO Yan;ZHANG Ya-feng;YIN Qing(Affiliated Hospital of Jiangsu University,Zhenjiang,Jiangsu 212000,China)

机构地区：[1]江苏大学附属医院检验科,江苏镇江212000 [2]江苏大学医学院,江苏镇江212013 [3]江苏大学附属医院感染管理科,江苏镇江212000

出　　处：《中华医院感染学杂志》2018年第18期2721-2724,2730,共5页Chinese Journal of Nosocomiology

基　　金：江苏省检验医学重点实验室开放基金资助项目(JSKLM-2014-020);镇江市科技创新基金资助项目(SH2017024)

摘　　要：目的研究鲍氏不动杆菌生物膜形成能力与耐药性的关系。方法收集2016年7月-2018年2月临床分离的85株鲍氏不动杆菌菌株,检测其药敏及生物膜形成能力,采用PCR技术检测鲍氏不动杆菌生物膜相关基因bap、abaI、bfs、bfmS、bfmR、csuA、csuE、csuAB。结果 85株鲍氏不动杆菌对替加环素、米诺环素、头孢哌酮/舒巴坦的耐药率分别为11.76%、23.53%、25.88%,耐药率最高的为环丙沙星和头孢吡肟,均为71.76%,其中耐药组(包括多重耐药和泛耐药菌)58株占68.24%,敏感组27株占31.76%;所有实验菌株均能形成生物膜,敏感组鲍氏不动杆菌生物膜形成能力强于耐药组细菌(Z=-6.391,P<0.001);85株鲍氏不动杆菌生物膜相关基因bfmS、bfmR、bfs、csuE、csuA、csuAB、abaI、bap的检出率分别为98.82%、98.82%、95.29%、90.59%、89.41%、89.41%、87.06%、78.82%,耐药组基因bap、abaI、bfs的检出率高于敏感组(P<0.05),其他基因在两组间检出率无差异。结论鲍氏不动杆菌耐药现象严重,敏感组细菌生物膜形成能力强于耐药组,多种基因参与细菌生物膜的形成。OBJECTIVE To study the relationship between the ability of biofilm formation and the drug resistance of Acinetobacter baumannii.METHODS A total of 85 clinical A.baumannii isolates were collected from Jul 2016 to Feb 2018,the drug susceptibility rates and the ability of biofilm formation of the strains were tested,and the biofilm-related genes of the A.baumannii strains such as bap,abaI,bfs,bfmS,bfmR,csuA,csuE and csuAB were detected by using PCR.RESULTS The drug resistance rates of the 85 strains of A.baumannii to tigecycline,minocycline and cefoperazone-sulbactam were 11.76%,23.53% and 25.88%,respectively;the drug resistance rates to ciprofloxacin and cefepime were the highest（71.76%）.There were 58（68.24%）strains of drug-resistant A.baumannii（including multidrug-resistant strains and pandrug-resistant strains）and 27（31.76%）strains of drug-sensitive A.baumannii.All of the test strains had the ability of biofilm formation,and the ability of biofilm formation of the drug-sensitive strains was greater than that of the drug-resistant strains（Z=-6.391,P〈0.001）.The detection rates of bfmS,bfmR,bfs,csuE,csuA,csuAB,abaI and bap were 98.82%,98.82%,95.29%,90.59%,89.41%,89.41%,87.06% and 78.82%,respectively.The detection rates of bap,abaI and bfs of the drug-resistant strains were significantly higher than those of the drug-sensitive strains（P〈0.05）;there were no significant differences in the detection rates of other genes between the two groups.CONCLUSIONThe A.baumannii strains are highly drug-resistant,the ability of biofilm formation of the drug-sensitive strains is greater than that of the drug-resistant strains,and multiple genes get involved in the formation of bacterial biofilm.

关 键 词：鲍氏不动杆菌 生物膜 多重耐药 基因 

分 类 号：R446.5[医药卫生—诊断学]