羟考酮预先给药对氧糖缺失-复氧复糖诱发大鼠小肠上皮细胞损伤的影响及不同阿片受体在其中的作用  

Effect of oxycodone pretreatment on oxygen-glucose deprivation and restoration-induced injury tosmall intestinal epithelial cells of rats and the role of different opioid receptors

作　　者：谢淑华 杨涛 耿立成[2] 王蕾[2] 王国林[1] Xie Shuhua, Yang Tao, Geng Licheng, Wang Lei, Wang Guolin,(Department of Anesthesiology, Tianjin Medical University General Hospital Tianjin Research Institute of Anesthesiology, Tianjin 300052, China （Xie SH, Wang GL ） ; Department of Anesthesiology, Tianjin Union Medical Center, Tianjin 300191, China)

机构地区：[1]天津医科大学总医院麻醉科天津市麻醉学研究所,300052 [2]天津市人民医院麻醉科,300191

出　　处：《中华麻醉学杂志》2018年第5期552-554,共3页Chinese Journal of Anesthesiology

摘　　要：目的探讨羟考酮预先给药对氧糖缺失-复氧复糖诱发大鼠小肠上皮细胞损伤的影响及不同阿片受体在其中的作用。方法采用随机数字表法，将IEC-6细胞分为5组（n=15）：正常对照组（C组）、氧糖缺失．复氧复糖组（OGD／R组）、羟考酮组（O组）、肛受体拮抗剂CTOP＋羟考酮组（CTOP＋O组）和K受体拮抗剂BNI＋羟考酮组（BNI＋O组）。c组正常条件下培养8h；OGD／R组、O组、CTOP＋O组和BNI＋O组采用无糖无血清培养液，置于95％N2-5％CO，条件下缺氧缺糖4h，再置于正常培养液中于常氧环境下复氧复糖4h。O组、CTOP＋O组和BNI＋O组于氧糖缺失前5min加入羟考酮，终浓度为1Ixg／ml。CTOP＋O组和BNI＋O组于氧糖缺失前10min分别加入CTOP和BNI，终浓度均为5μmol／L。于复氧复糖4h时每组取5孔，采用MTI＂法测定细胞活力；采用比色法测定培养液LDH漏出率；采用ELISA法测定培养液上清TNF-α、IL-1β和高迁移率族蛋白Bl（HMGB1）的水平。结果与C组比较，OGD／R组、O组、CTOP＋O组和BNI＋O组细胞活力降低．LDH漏出率、TNF-α、IL-1β和HMGB1的水平升高（P〈0．05）；与OGD／R组比较，O组细胞活力升高，LDH漏出率、TNF-α、IL-1β和HMGB1的水平降低（P〈0．05）；与O组比较，CTOP＋O组和BNI＋O组细胞活力降低，LDH漏出率、TNF-α、IL-1β和HMGB1的水平升高（P〈0．05）。结论羟考酮预先给药可减轻氧糖缺失-复氧复糖诱发的大鼠小肠上皮细胞损伤，其机制与激活肛受体和K受体有关。Objective To evaluate the effect of oxycodone pretreatment on oxygen-glucose deprivation and restoration （OGD/R） -induced injury to small intestinal epithelial cells of rats and the role of different opioid receptors. Methods IEC-6 cells were divided into 5 groups （n= 15 each） using a random num- ber table： control group （group C） , group OGD/R, oxycodone group （group O） , tz opioid receptor an- tagonist CTOP plus oxycodone group （group CTOP＋O） and K opioid receptor antagonist BNI plus oxycodone group （group BNI＋O）. Cells were cultured for 8 h in normal culture atmosphere in group C. Cells were subjected to OGD for 4 h followed by restoration of oxygen-glucose supply in normal culture medium for 4 h in OGD/R, O, CTOP＋O and BNI＋O groups. Oxycodone at a final concentration of 1 μg/ml was added at 5 rain before OGD/R in O, CTOP＋O and BNI＋O groups. CTOP and BNI at a final concentration of 5 μmol/L were added at 10 min before OGD/R in group CTOP＋O and group BNI＋O, respectively. Five holes in eachgroup were selected at 8 h of OGD/R for determination of the cell viability （by MTF assay） , lactic dehydro- genase （LDH） release rate （by colorimetry） and levels of tumor necrosis factor-alpha （TNF-α） , interleu- kin-lbeta （IL-1β） and high-mobility group box 1 protein （HMGB1） in supernatant （by enzyme-linked im- munosorbent assay）. Results Compared with group C, the cell viability was significantly decreased, and the LDH release rate and levels of TNF-α, IL-1β and HMGB1 were increased in OGD/R, O, CTOP＋O and BNI＋ O groups （P〈 0. 05 ）. Compared with group OGD/R, the cell viability was significantly in- creased, and the LDH release rate and levels of TNF-c~, IL-113 and HMGB1 were decreased in group O （P〈0.05）. Compared with group O, the cell viability was significantly decreased, and the LDH release rate and levels of TNF-α, IL-1β and HMGB1 were increased in CTOP＋O and BNI＋O groups （P〈0. 05）. Conehmion Oxycodone pretreatment

关 键 词：羟可酮 再灌注损伤 上皮细胞 肠 受体 阿片样 

分 类 号：R614[医药卫生—麻醉学]