哺乳动物睾丸蛋白质组学研究进展  

Research Progress on Proteomics of Mammalian Testis

作　　者：孙武 罗静 王丽 李发弟 李万宏 罗莉宁 乐祥鹏 SUN WU;LUO Jing;WANG Li;LI Fa-Di;LI Wan-Hong;LUO Li-Ning;YUE Xiang-Peng(State Key Laboratory of Grassland Agro-ecosystems/Key Laboratory of Grassland Livestock Industry Innovation,Ministry of Agriculture and Rural Affairs,College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,China;Sheep Breeding Biotechnology Engineering Laboratory of Gansu Province,Minqin 733300,China;Gansu Provincial Centre for Animal Disease Control and Prevention,Lanzhou 730046,China)

机构地区：[1]兰州大学草地农业科技学院草地农业生态系统国家重点实验室/农业农村部草牧业创新重点实验室,兰州730020 [2]甘肃省肉羊繁育生物技术工程实验室,民勤733300 [3]甘肃省动物疾病预防控制中心,兰州730046

出　　处：《农业生物技术学报》2018年第10期1797-1804,共8页Journal of Agricultural Biotechnology

基　　金：国家科技支撑计划(No.2015BAD03B05);甘肃省科技重大专项计划(No.1602NKDH020);国家肉羊产业技术体系(CARS-38);甘肃省农业生物技术专项(GNSW-2015-24; GNSW-2016-22);甘肃省农业科技创新项目(GNCX-2014-41);长江学者和创新团队发展计划资助(No.IRT13019)

摘　　要：睾丸具有生精、分泌雄激素等重要功能,是雄性哺乳动物特有的生殖器官,其正常的结构和生理机能是繁殖活动的基础。蛋白质组学是后基因组时代蛋白质组研究的主要方法之一,可以反映细胞或者组织整体的蛋白质动态变化。因此,应用蛋白质组学技术解析睾丸中蛋白质的组成意义重大。本文就蛋白质组学研究技术手段及其在动物睾丸发育、疾病等方面的应用进行综述,旨在为下一步研究控制睾丸发育和大小的基因和调控网络提供参考依据。The testes are the most important reproductive organs in male mammals as they are the site of testosterone production and spermatogenesis. Normal testicular structure and physiology are the basis for reproductive activity. Proteomics is one of main methods to study proteome at post-genomics era, which may reflect the dynamic nature of proteins in whole tissue or cell. Thus it is of great significance to explore the protein compositions of testis by proteomics techniques. In this review, we mainly focus on the technological means of quantitative proteomics, as well as its application in the studies of testicular development, and responses to diseases conditions, which provides insight into the further research on genes and regulatory networks that control testicular development and size.

关 键 词：蛋白质组学 哺乳动物 睾丸 

分 类 号：S814.1[农业科学—畜牧学]