头穴针刺调控局灶性脑缺血大鼠海马旁回NF-κB p 65 mRNA、IκB mRNA表达的实验研究  

Scalp-acupuncture Improves Neurological Function by Regulating Expression of NF-κB p 65mRNA,IκB mRNA and IL-1β and TNF-α in Parahippocampal Gyrus of Cerebral Ischemia Rats

作　　者：王金海[1] 张婷卓 赵敏[1] 鲍英存[1] 鄢琦[3] 张振昶[3] 杜小正 姜华[1] 田亮 袁博 李兴兰 WANG Jin-hai;ZHANG Ting-zhuo;ZHAO Min;BAO Ying-cun;YAN Qi;ZHANG Zhen-chang;DU Xiao-zheng;JIANG Hua;TIAN Liang;YUAN Bo;LI Xing-lan(Department of Traditional Chinese Medicine,Second Hospital of Lanzhou University,Lanzhou 730030,China;School of Acupuncture-moxibustion and Massage,Gansu University of Tradi-tional Chinese Medicine,Lanzhou 730000;Department of Neurology,Second Hospital of Lanzhou University,Lanzhou 730030)

机构地区：[1]兰州大学第二医院中医科,兰州730030 [2]甘肃中医药大学针灸推拿学院,兰州730000 [3]兰州大学第二医院神经内科,兰州730030

出　　处：《针刺研究》2018年第9期556-561,共6页Acupuncture Research

基　　金：兰州大学第二医院院内博士科研基金(No.2015-1-26)

摘　　要：目的:观察头穴针刺对局灶性脑缺血大鼠海马旁回核因子(NF)-κB p 65mRNA、IκB mRNA及白介素(IL)-1β、肿瘤坏死因子(TNF)-α表达的影响,探讨头穴针刺调控缺血性脑血管病炎性反应的机制。方法:SD大鼠随机分为正常组、模型组、药物组及头针组,每组16只。采用线栓法建立大鼠急性局灶性脑缺血模型。药物组腹腔注射吡咯烷二硫代氨基甲酸铵盐(100mg·kg^(-1)·d^(-1)),1次/d,共7d。头针组取双侧顶颞前斜线和顶颞后斜线行快速捻转透刺治疗,每次30min,1次/d,共7d。干预前后进行神经功能缺损评分及神经行为学评分。RT-PCR法检测海马旁回NF-κB p 65mRNA、IκB mRNA的表达,免疫组织化学法检测海马旁回IL-1β、TNF-α的表达。结果:与正常组比较,模型组大鼠神经功能缺损评分和神经行为学评分明显增高(P<0.01),海马旁回NF-κB p 65 mRNA、IL-1β、TNF-α的表达均明显增高(P<0.01),IκB mRNA表达显著降低(P<0.01)。与模型组比较,头针组和药物组大鼠神经功能缺损评分和神经行为学评分明显降低(P<0.01),海马旁回NF-κB p 65 mRNA、IL-1β、TNF-α表达均明显降低(P<0.05),IκB mRNA表达明显增高(P<0.05)。结论:上调缺血后脑组织IκB的表达,抑制NF-κB的解离,进而降低炎性因子IL-1β、TNF-α的表达是头穴针刺控制缺血性脑血管病炎性反应的分子机制之一。Objective To observe the effect of scalp-acupuncture intervention on the expression of parahippocampal factor-κB p 65 mRNA （NF-κB p 65 mRNA）, IκB mRNA, interleukin-1 β（IL-1β） and tumor necrosis factor-α（TNF-α） in rats with cerebral ischemia （CI）, so as to investigate its molecular mechanisms underlying improving CI by reducing inflammatory response. Methods A total of 64 SD rats in each group. The foca rats were randomized into normal control, model, medication and scalp-acupuncture groups, with 16 CI model was established by middle cerebral artery occlusion （MCAO）. Intraperitoneal injection of Pyrrolidine Dithiocarbamate （100 mg o kg-1 o d-1） was administrated for rats in the medication group, once a day for 7 days. For rats of the scalp-acupuncture group, the acupuncture needles were rapidly inserted into bilateral Dingnieqianxiexian （MS 6） and Dingniehouxiexian （MS 7）, followed by twirling the needles at 200 cycles/min for 1 min, once again every 10 rain during 30 min＇s needle retention. The treatment was conducted once a day for 7 days. The neurologic deficit score （0- 5 points, impaired con- sciousness, death, etc. ） and neurological function score （motor, sensory and sensory tests, 0- 10 points） were assessed according to Longa＇s （1989） and Sch^bitz＇s （2004） methods, respectively. The expression levels of NF-κB p 65 mRNA and IκB mRNA in the parahippocampus gyrus tissue were detected by fluorescence quantitative reverse transcription-POR, and IL-1 β and TNF-α proteins in the parahippocampus gyrus tissue were detected by immunohistochemistry. Results After modeling, the neu- rologic deficit and neurological function scores and the expression levels of NF-κB p 65 mRNA, IL-1 β and TNF-α in the parahip- pocampus were significantly increased in the model group than in the normal group （P〈0.01）, while the expression of IκB mRNA was considerably down-regulated （ P〈 0.01 ）. Following treatment intervention, the neurologic deficit and ne

关 键 词：头针 急性缺血性脑血管病 神经功能缺损 海马旁回 核因子-κB P 65 MRNA IΚB MRNA 白介素-1β 肿瘤坏死因子-α 

分 类 号：R246.6[医药卫生—针灸推拿学]